Department of Medical Histology and Cell Biology, Faculty of Medicine, Mansoura University, Mansoura, Egypt.
Department of Anatomy, Taibah College of Medicine, Taibah University, Taibah, Saudi Arabia.
Int J Exp Pathol. 2019 Oct;100(5-6):369-377. doi: 10.1111/iep.12343. Epub 2020 Feb 10.
Empagliflozin (EMPA) is a promising novel antidiabetic drug; however, doubts have been raised regarding its use and the increased risk of urinary bladder carcinoma. In this study, we evaluated urothelium expression of cytokeratins (CKs) and Ki-67 proliferative activity in the urinary bladder of diabetic (DM + EMPA) and non-diabetic rats after EMPA administration. By routine histology, dysplastic changes were detected in the urothelium of diabetic as well as non-diabetic animals after EMPA administration. Moreover, the expression of CK-7 and CK-8 was significantly decreased (P < .05) while that of CK-20 as well as Ki-67 was significantly increased (P < .05) in EMPA per se and DM + EMPA urothelium groups compared to that of control and diabetics. The dysplastic changes together with the increased proliferative activity in urothelium after EMPA administration provide a cellular evidence that supports the former clinical concerns.
恩格列净（EMPA）是一种有前途的新型抗糖尿病药物；然而，人们对其使用和膀胱癌风险增加表示怀疑。在这项研究中，我们评估了 EMPA 给药后糖尿病（DM+EMPA）和非糖尿病大鼠膀胱的细胞角蛋白（CKs）表达和 Ki-67 增殖活性。通过常规组织学，在 EMPA 给药后，糖尿病和非糖尿病动物的尿路上皮均检测到发育不良改变。此外，与对照组和糖尿病组相比，EMPA 本身和 DM+EMPA 尿路上皮组中 CK-7 和 CK-8 的表达显著降低（P<.05），而 CK-20 和 Ki-67 的表达显著增加（P<.05）。EMPA 给药后尿路上皮的发育不良改变和增殖活性增加为支持先前临床关注的细胞证据提供了依据。
