State Key Laboratory of Crop Stress Biology in Arid Areas, College of Plant Protection, Northwest A&F University, Yangling 712100, China.
State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China.
J Agric Food Chem. 2020 Mar 4;68(9):2711-2717. doi: 10.1021/acs.jafc.9b07305. Epub 2020 Feb 20.
is a global pest of stone and pome fruits. The sensitive olfactory system plays a crucial role in regulating key behavioral activities of insects and relies heavily on general odorant receptors (ORs) to detect host-plant volatiles. In this study, three general OR genes from (, , and ) were identified. Quantitative polymerase chain reaction revealed that expression was considerably higher in adults and adult antennae than in any other life stages and body parts, respectively. Moreover, the expression of was significantly higher in the antennae of females than in those of males, with a peak in the antennae of 3-days-old adult females. GmolOR20 and GmolOR21 displayed no responses to any of the odorant compounds tested in the oocyte system. GmolOR12 was tuned mainly to 5 of the 47 odorant components tested (including decanol, heptanal, octanal, nonanal, and decanal), and the response to aldehydes among the 5 components was the highest. Additionally, they all elicited female and male antennae electroantennogram responses, and the aldehydes elicited the highest response among the 5 components. These results suggested that in the olfactory system plays an important role in sensing aldehydes and that is involved in sensing host-plant volatiles. These findings provide insight into the possibility of using host-plant volatiles for the control of .
是一种全球性的石果和苹果害虫。敏感的嗅觉系统在调节昆虫的关键行为活动中起着至关重要的作用，并且严重依赖于一般气味受体（ORs）来检测寄主植物挥发物。在这项研究中，从 （ ， ，和 ）中鉴定出了三个一般的 OR 基因。定量聚合酶链反应显示， 表达在成虫和成虫触角中明显高于其他任何生命阶段和身体部位。此外， 在雌虫触角中的表达明显高于雄虫，在 3 天龄雌虫触角中达到峰值。GmolOR20 和 GmolOR21 在卵母细胞系统中对测试的任何气味化合物都没有反应。GmolOR12 主要对测试的 47 种气味成分中的 5 种（包括癸醇、庚醛、辛醛、壬醛和癸醛）有反应，而对 5 种成分中的醛类的反应最高。此外，它们都能引起雌、雄触角的电触角反应，而醛类在 5 种成分中引起的反应最高。这些结果表明， 在 嗅觉系统中对醛类的感知起着重要作用，并且 参与了寄主植物挥发物的感知。这些发现为利用寄主植物挥发物控制 提供了可能性。
