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RNA测序揭示了……对不同宿主的潜在适应机制。 （注：原文不完整，句末有省略内容）

RNA Sequencing Reveals the Potential Adaptation Mechanism to Different Hosts of .

作者信息

Lü Dongbiao, Yan Zizheng, Hu Di, Zhao Aiping, Wei Shujun, Wang Ping, Yuan Xiangqun, Li Yiping

机构信息

Key Laboratory of Integrated Pest Management on Crops in Northwestern Loess Plateau, Ministry of Agriculture, College of Plant Protection, Northwest A&F University, Yangling, Xianyang 712100, China.

Institute of Plant Protection, Beijing Academy of Agriculture and Forestry Sciences, 9 Shuguanghuayuan Middle Road, Haidian District, Beijing 100097, China.

出版信息

Insects. 2022 Sep 30;13(10):893. doi: 10.3390/insects13100893.

DOI:10.3390/insects13100893
PMID:36292841
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9604371/
Abstract

is an important fruit tree worldwide pest which feeds on hosts extensively and does serious harm. In this paper, the growth and development parameters and protease activities of fed on different hosts were compared. Using Illumina RNA sequencing technology, 18 midgut samples from five different hosts (apple, pear, plum, peach and peach shoots) and artificial diet were sequenced and compared with the reference genome, resulting in 15269 genes and 2785 predicted new genes. From 15 comparative combinations, DEGs were found from 286 to 4187 in each group, with up-regulated genes from 107 to 2395 and down-regulated genes from 83 to 2665. KEGG pathway analysis showed that DEGs were associated with amino acid metabolism, starch and sucrose metabolism, carbohydrate metabolism, and hydrolase activity. A total of 31 co-expression gene modules of different hosts were identified by WGCNA. qRT-PCR showed that the expression pattern of the trypsin gene was consistent with RNA sequencing. In this study, growth and development parameters, protease activity, DEGs, enrichment analysis and qRT-PCR were combined to reveal the adaptation process to different hosts of in many aspects. The results of this study provide a basis for further exploration of the molecular mechanism of host adaptation of

摘要

是一种重要的世界性果树害虫，寄主广泛，危害严重。本文比较了取食不同寄主的[害虫名称未给出]的生长发育参数和蛋白酶活性。利用Illumina RNA测序技术，对来自五种不同寄主（苹果、梨、李、桃和桃梢）以及人工饲料的18个中肠样本进行测序，并与参考基因组进行比较，共得到15269个基因和2785个预测的新基因。在15个比较组合中，每组发现的差异表达基因（DEG）从286个到4187个不等，上调基因从107个到2395个，下调基因从83个到2665个。KEGG通路分析表明，差异表达基因与氨基酸代谢、淀粉和蔗糖代谢、碳水化合物代谢以及水解酶活性有关。通过加权基因共表达网络分析（WGCNA）共鉴定出不同寄主的31个共表达基因模块。定量逆转录聚合酶链反应（qRT-PCR）表明，胰蛋白酶基因的表达模式与RNA测序结果一致。本研究结合生长发育参数、蛋白酶活性、差异表达基因、富集分析和qRT-PCR，从多个方面揭示了[害虫名称未给出]对不同寄主的适应过程。本研究结果为进一步探索[害虫名称未给出]寄主适应性的分子机制提供了依据

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3d8b/9604371/4fdcee2f8e6e/insects-13-00893-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3d8b/9604371/88613a8d06a7/insects-13-00893-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3d8b/9604371/97b05669d9ad/insects-13-00893-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3d8b/9604371/be8061596cd4/insects-13-00893-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3d8b/9604371/1abca12cf138/insects-13-00893-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3d8b/9604371/dda4d4f306da/insects-13-00893-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3d8b/9604371/d64afcb139c6/insects-13-00893-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3d8b/9604371/528eeb18d3d8/insects-13-00893-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3d8b/9604371/a8b63892fe2e/insects-13-00893-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3d8b/9604371/4fdcee2f8e6e/insects-13-00893-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3d8b/9604371/88613a8d06a7/insects-13-00893-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3d8b/9604371/97b05669d9ad/insects-13-00893-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3d8b/9604371/be8061596cd4/insects-13-00893-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3d8b/9604371/1abca12cf138/insects-13-00893-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3d8b/9604371/dda4d4f306da/insects-13-00893-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3d8b/9604371/d64afcb139c6/insects-13-00893-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3d8b/9604371/528eeb18d3d8/insects-13-00893-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3d8b/9604371/a8b63892fe2e/insects-13-00893-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3d8b/9604371/4fdcee2f8e6e/insects-13-00893-g009.jpg

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