Gokmen Aysegul A, Can Hüseyin, Kayalar Hüsniye, Pektaş Bayram, Kaya Selçuk
Department of Microbiology, Faculty of Medicine, İzmir Katip Çelebi University, İzmir, Turkey.
Molecular Biology Section, Department of Biology, Faculty of Science, Ege University, İzmir, Turkey.
J Infect Dev Ctries. 2019 Mar 31;13(3):240-244. doi: 10.3855/jidc.10854.
In the classic treatment of Trichomonas vaginalis infection, although metronidazole has been used since the 1960s, there has been an increase in MTZ-resistant T. vaginalis strains and failure in the treatment of trichomoniasis causes serious concerns. Therefore, the present study aimed to investigate the in vitro antitrichomonal activities of extracts (ethanol and total alkaloid) and pure compounds (chrysosplenetin, dictamnine, gamma-Fagarine, skimmianine) of H. myrtifolium against T. vaginalis.
H. myrtifolium was collected from the town of Honaz in Denizli, located in the Aegean region of Turkey, and preparation of extracts and isolation and structure elucidation of pure compounds were performed. Later, different concentrations of extracts and pure compounds were incubated with T. vaginalis trophozoites isolated from Turkey, which are known to be sensitive to metronidazole.
It was found that ethanol extract caused a more effective lysis on T. vaginalis trophozoites compared with total alkaloid extract (P < 0.05). No compounds except for furoquinoline alkaloid skimmianine prepared above 37.5 µg/mL were found to have any inhibitory effect on T. vaginalis trophozoites.
The ethanol extract of H. myrtifolium and skimmianine can be considered as potential candidates for antitrichomonal drug development.
在经典的阴道毛滴虫感染治疗中，尽管甲硝唑自20世纪60年代就已被使用，但对甲硝唑耐药的阴道毛滴虫菌株有所增加，滴虫病治疗失败引发了严重关注。因此，本研究旨在探究桃金娘叶山小橘提取物（乙醇提取物和总生物碱）及纯化合物（金橘素、白鲜碱、γ-崖椒碱、茵芋碱）对阴道毛滴虫的体外抗滴虫活性。
从位于土耳其爱琴海地区代尼兹利省霍纳兹镇采集桃金娘叶山小橘，进行提取物制备以及纯化合物的分离和结构解析。随后，将不同浓度的提取物和纯化合物与从土耳其分离出的已知对甲硝唑敏感的阴道毛滴虫滋养体共同孵育。
发现乙醇提取物对阴道毛滴虫滋养体的裂解作用比总生物碱提取物更有效（P < 0.05）。除了上述浓度高于37.5 µg/mL制备的呋喃喹啉生物碱茵芋碱外，未发现其他化合物对阴道毛滴虫滋养体有任何抑制作用。
桃金娘叶山小橘乙醇提取物和茵芋碱可被视为抗滴虫药物开发的潜在候选物。
