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白藜芦醇可减轻尿毒症大鼠的肾损伤，其作用与热休克蛋白 70（Hsp70）表达增加有关。

Resveratrol Reduces Kidney Injury in a Rat Model of Uremia and is Associated with Increased Expression of Heat Shock Protein 70 (Hsp70).

机构信息

Hemodialysis Room, Yantaishan Hospital, Yantai, Shandong, China (mainland).

Department of Anesthesiology, Qingdao Central Hospital, Qingdao, Shandong, China (mainland).

出版信息

Med Sci Monit. 2020 Feb 10;26:e919086. doi: 10.12659/MSM.919086.

DOI:10.12659/MSM.919086
PMID:32040471
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7032531/
Abstract

BACKGROUND This study aimed to investigate the effects of resveratrol on kidney function in a rat model of uremia and the expression of heat shock proteins. MATERIAL AND METHODS The rat model of uremia was developed by 5/6 nephrectomy of Sprague-Dawley rats. The Hsp70 inhibitor MKT-077, a rhodacyanine dye, was used. The study groups included rats with sham surgery (the sham group), the rat model of uremia (the model group), the solvent-treated control group (the control group), the rat model treated with resveratrol group (the resveratrol group), the rat model treated with MKT-077 (the MKT-077 group), and the resveratrol+MKT-077 group. Kidney tissues were studied histologically. Renal cell apoptosis was detected by the TUNEL method. Expression of p53, Bax, and Bcl-2 mRNA and protein were detected by quantitative reverse transcription-polymerase chain reaction (qRT-PCR) and immunohistochemistry, respectively. RESULTS Compared with the sham group, the expression levels of heat shock proteins Hsp70, Hsp90, Hsp27, Hsp25, Hsp40, and Hsp60 in the kidney of the rat model group increased to different degrees. Compared with the model group, the Hsp70 levels in the resveratrol group were significantly increased (p<0.05). Compared with the model group, treatment with MKT-077 reduced the survival rate of rats, which was increased following resveratrol treatment. Compared with the resveratrol group, renal function in the resveratrol+MKT-077 group was significantly reduced (p<0.05). CONCLUSIONS In a rat model of uremia, resveratrol reduced renal injury and improved both renal function and survival, which were associated with increased expression of Hsp70.

摘要

背景

本研究旨在探讨白藜芦醇对尿毒症大鼠模型肾功能及热休克蛋白表达的影响。

材料和方法

通过 Sprague-Dawley 大鼠 5/6 肾切除术建立尿毒症大鼠模型。使用热休克蛋白 70 抑制剂 MKT-077（一种罗丹明染料）。研究组包括假手术大鼠（假手术组）、尿毒症大鼠模型（模型组）、溶剂处理对照组（对照组）、白藜芦醇处理组（白藜芦醇组）、MKT-077 处理组（MKT-077 组）和白藜芦醇+MKT-077 组。研究了肾组织的组织学变化。通过 TUNEL 法检测肾细胞凋亡。通过定量逆转录聚合酶链反应（qRT-PCR）和免疫组织化学法分别检测 p53、Bax 和 Bcl-2mRNA 和蛋白的表达。

结果

与假手术组相比，模型组大鼠肾脏中热休克蛋白 Hsp70、Hsp90、Hsp27、Hsp25、Hsp40 和 Hsp60 的表达水平均不同程度升高。与模型组相比，白藜芦醇组 Hsp70 水平显著升高（p<0.05）。与模型组相比，MKT-077 处理降低了大鼠的存活率，而白藜芦醇处理后存活率增加。与白藜芦醇组相比，白藜芦醇+MKT-077 组的肾功能明显降低（p<0.05）。

结论

在尿毒症大鼠模型中，白藜芦醇可减轻肾损伤，改善肾功能和存活率，这与 Hsp70 的表达增加有关。

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