褪黑素在预防年龄相关性听力损失中的作用。
Role of melatonin in prevention of age-related hearing loss.
机构信息
Laboratory of Otorhinolaryngology Research, University of Brasilia, Brasília, Distrito Federal, Brazil.
Department of Tropical Medicine, University of Brasilia, Brasília, Distrito Federal, Brazil.
出版信息
PLoS One. 2020 Feb 10;15(2):e0228943. doi: 10.1371/journal.pone.0228943. eCollection 2020.
INTRODUCTION
Age-related hearing loss (ARHL) is a consequence of aging of the auditory system. The best known mechanism of cell death in ARHL is apoptosis due to increased production of reactive oxygen species. In this context, it is hypothesized that melatonin, owing to its high antioxidant potential and its action in the mitochondria, helps prevent or delay outer hair cell dysfunction (HCD).
AIMS
To evaluate the effect of melatonin on the prevention of HCD dysfunction in the ARHL process in a susceptible murine C57BL/6J model.
METHOD
C57BL/6J animals were divided into two groups: control (CG) and melatonin (MG). The CG received a saline and ethanol solution and the MG, melatonin (10 mg/kg/day). The solutions were offered daily (50 μl) orally over a 10-month period. Distortion Product Otoacoustic Emissions (DPOAE) measurements were conducted once a month.
RESULTS
There was a decrease in DPOAE values in both groups over time and a differentiation between them from the 10th month of life onwards. At 10 months, the MG maintained higher DPOAE values than the CG at all frequencies tested.
CONCLUSION
The use of melatonin has otoprotective effects on HCD in the ARHL process in the C57BL/6J model.
简介
年龄相关性听力损失（ARHL）是听觉系统老化的结果。ARHL 中细胞死亡的最知名机制是由于活性氧物质产生增加而导致的细胞凋亡。在这种情况下，有人假设褪黑素由于其高抗氧化潜力及其在线粒体中的作用，有助于预防或延迟外毛细胞功能障碍（HCD）。
目的
评估褪黑素对易感 C57BL/6J 模型 ARHL 过程中外毛细胞功能障碍（HCD）的预防作用。
方法
将 C57BL/6J 动物分为两组：对照组（CG）和褪黑素组（MG）。CG 接受盐水和乙醇溶液，MG 接受褪黑素（10 mg/kg/天）。这些溶液每天（50 μl）通过口服提供，持续 10 个月。每月进行一次畸变产物耳声发射（DPOAE）测量。
结果
随着时间的推移，两组的 DPOAE 值均下降，并且从生命的第 10 个月开始，两组之间出现分化。在 10 个月时，MG 在所有测试频率下的 DPOAE 值均高于 CG。
结论
褪黑素的使用对 C57BL/6J 模型的 ARHL 过程中的 HCD 具有耳保护作用。
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