Department of Chemistry, Faculty of Science, Ain Shams University, Cairo 11566, Egypt.
Chemistry Department, College for Women, Ain Shams University, Heliopolis, Cairo 11751, Egypt.
Biomolecules. 2020 Feb 6;10(2):251. doi: 10.3390/biom10020251.
Potentiometric sensors have a great influence on the determination of most various compounds in their matrices. Therefore, efficient and new sensors were introduced to measure sodium Deoxycholate (NaDC) as a bile acid salt. These sensors are based on NaDC imprinted polymer (MIP) as sensory element. The MIP beads were synthesized using thermal polymerization pathway, in which acrylamide (AAm), ethylene glycol dimethacrylate (EGDMA), NaDC, and benzoyl peroxide (BPO) were used as the functional monomer, cross-linker, template, and initiator, respectively. The proposed sensors were fabricated using a coated screen-printed platform and the sensing membrane was modified by single-walled carbon nanotubes (SWCNTs) as an ion-to-electron transducer. The sensors exhibited high sensitivity that reached 4.7 × 10 M of near-Nernestian slope (-60.1 ± 0.9 mV/decade, = 0.999 (= 5)). In addition, the sensors revealed high selectivity, long lifetime, high potential stability, and conductivity that ensure reproducible and accurate results over a long time. MIP characterization was performed using Fourier Transform-Infrared (FT-IR) and a scanning electron microscope (SEM). Regarding the interaction of NaDC with serum albumin (SA), albumin is determined in human serum samples as human serum albumin (HSA), which was collected from different volunteers of different ages and gender.
电位传感器对其基质中大多数各种化合物的测定有很大的影响。因此，引入了高效的新型传感器来测量胆酸钠盐（NaDC）。这些传感器基于 NaDC 印迹聚合物（MIP）作为敏感元件。MIP 珠粒是通过热聚合途径合成的，其中丙烯酰胺（AAm）、乙二醇二甲基丙烯酸酯（EGDMA）、NaDC 和过氧化苯甲酰（BPO）分别用作功能单体、交联剂、模板和引发剂。所提出的传感器是使用涂覆的丝网印刷平台制造的，传感膜通过单壁碳纳米管（SWCNTs）进行修饰，作为离子到电子的转换器。传感器表现出高灵敏度，达到近 Nernestian 斜率的 4.7×10 M（-60.1±0.9 mV/decade， = 0.999（ = 5））。此外，传感器表现出高选择性、长寿命、高电位稳定性和电导率，确保在长时间内可重复和准确的结果。使用傅里叶变换红外（FT-IR）和扫描电子显微镜（SEM）对 NaDC 与血清白蛋白（SA）的相互作用进行了表征。关于 NaDC 与血清白蛋白（SA）的相互作用，白蛋白在人血清样品中被确定为人血清白蛋白（HSA），其是从不同年龄和性别的不同志愿者收集的。
