Hassan Saad S M, H Kamel Ayman, Amr Abd El-Galil E, Hashem Heba M, Bary E M Abdel
Chemistry Department, Faculty of Science, Ain Shams University, Abbasia, Cairo 11566, Egypt.
Pharmaceutical Chemistry Department, Drug Exploration & Development Chair (DEDC), College of Pharmacy, King Saud University, Riyadh 11451, Saudi Arabia.
Nanomaterials (Basel). 2020 Mar 21;10(3):572. doi: 10.3390/nano10030572.
Herein, we present a new validated potentiometric method for fluoxetine (FLX) drug monitoring. The method is based on the integration of molecular imprinting polymer (MIP) beads as sensory elements with modified screen-printed solid contact ion-selective electrodes (ISEs). A multi-walled carbon nanotube (MWCNT) was used as a nanomaterial for the ion-to-electron transduction process. The prepared MIP beads depend on the use of acrylamide (AAm) and ethylene glycol dimethacrylic acid (EGDMA) as a functional monomer and cross-linker, respectively. The sensor revealed a stable response with a Nernstian slope of 58.9 ± 0.2 mV/decade and a detection limit of 2.1 × 10 mol/L in 10 mmol/L acetate buffer of pH 4.5. The presented miniaturized sensors revealed good selectivity towards FLX over many organic and inorganic cations, as well as some additives encountered in the pharmaceutical preparations. Repeatability, reproducibility and stability have been studied to evaluate the analytical features of the presented sensors. These sensors were successfully applied for FLX assessment in different pharmaceutical formulations collected from the Egyptian local market. The obtained results agreed well with the acceptable recovery percentage and were better than those obtained by other previously reported routine methods.
在此，我们提出了一种新的经过验证的电位法用于氟西汀（FLX）药物监测。该方法基于将分子印迹聚合物（MIP）微球作为传感元件与修饰的丝网印刷固体接触离子选择电极（ISE）相结合。多壁碳纳米管（MWCNT）用作离子到电子转换过程的纳米材料。制备的MIP微球分别依赖于使用丙烯酰胺（AAm）和乙二醇二甲基丙烯酸酯（EGDMA）作为功能单体和交联剂。该传感器在pH 4.5的10 mmol/L醋酸盐缓冲液中显示出稳定的响应，能斯特斜率为58.9±0.2 mV/十倍，检测限为2.1×10 mol/L。所展示的微型传感器对FLX表现出良好的选择性，优于许多有机和无机阳离子以及药物制剂中遇到的一些添加剂。研究了重复性、再现性和稳定性以评估所展示传感器的分析特性。这些传感器成功应用于从埃及当地市场收集的不同药物制剂中FLX的评估。获得的结果与可接受的回收率百分比吻合良好，并且优于其他先前报道的常规方法所获得的结果。
