伊朗甜瓜地方品种内部及之间的种群结构、形态和遗传多样性。

Population structure, morphological and genetic diversity within and among melon ( L.) landraces in Iran.

作者信息

Maleki Masoud, Shojaeiyan Abdolali, Rashidi Monfared Sajad

机构信息

Department of Horticultural Science, Faculty of Agriculture, Tarbiat Modares University, Tehran, Iran.

Department of Agricultural Biotechnology, Faculty of Agriculture, Tarbiat Modares University, Tehran, Iran.

出版信息

J Genet Eng Biotechnol. 2018 Dec;16(2):599-606. doi: 10.1016/j.jgeb.2018.08.002. Epub 2018 Aug 28.

DOI:10.1016/j.jgeb.2018.08.002

PMID:30733778

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6353764/

Abstract

ISSR markers were applied to evaluate the genetic diversity and differentiation of 270 individuals of 27 Iranian landraces of various varietal groups include vars. , . Genetic diversity among the studied genotypes obtained by GeneAlex analysis ( = 0.08,  = 0.12,  = 0.77,  = 22.6%). Cluster analysis divided Iranian melon landraces into two main cluster. Non-sweet genotype ( group) was well separated from sweet genotypes (, , , ). The most similar genotypes were BANI and TONI (0.95) and the most dissimilar ones were GER and TS (0.58). AMOVA result showed that the percentage of genetic variation among and within Iranian melon is 69% and 31%, respectively. All landraces evaluated based on 10 morphological traits which revealed the diversity of melon varietal groups. Bayesian analysis assigned ten landraces to Pop 1, eight landraces to Pop 2 and nine melon landraces to Pop 3. Bayesian and UPGMA cluster analyses demonstrated the almost related results. Our results indicated that ISSR markers technique alongside polyacrylamide gel analysis could be helpful to discriminate varieties of melon.

摘要

ISSR标记被用于评估27个伊朗地方品种的270个个体的遗传多样性和分化，这些地方品种属于不同的品种组，包括变种……通过GeneAlex分析获得的研究基因型之间的遗传多样性（等位基因数 = 0.08，有效等位基因数 = 0.12，Nei's基因多样性指数 = 0.77，Shannon信息指数 = 22.6%）。聚类分析将伊朗甜瓜地方品种分为两个主要聚类。非甜基因型（组）与甜基因型（……）明显分开。最相似的基因型是BANI和TONI（0.95），最不相似的是GER和TS（0.58）。AMOVA结果表明，伊朗甜瓜群体间和群体内的遗传变异百分比分别为69%和31%。基于10个形态性状对所有地方品种进行评估，揭示了甜瓜品种组的多样性。贝叶斯分析将10个地方品种归入种群1，8个地方品种归入种群2，9个甜瓜地方品种归入种群3。贝叶斯分析和UPGMA聚类分析显示了几乎相关的结果。我们的结果表明，ISSR标记技术与聚丙烯酰胺凝胶分析相结合有助于区分甜瓜品种。