2018 年缅甸无症状感染者中的 Zika 病毒感染。
Zika virus infection in asymptomatic persons in Myanmar, 2018.
机构信息
Department of Virology, Institute of Tropical Medicine, Nagasaki University, 1-12-4 Sakamoto, Nagasaki, Japan, PO 852-8523.
Department of Pathology, University of Medicine-1, No. 245, Lanmadaw township, Yangon, Myanmar, PO 11131.
出版信息
Trans R Soc Trop Med Hyg. 2020 Jun 1;114(6):440-447. doi: 10.1093/trstmh/trz134.
BACKGROUND
Zika virus (ZIKV) is a mosquito-borne flavivirus. Outbreaks of ZIKV infection have occurred in Africa, Southeast Asia, the Pacific Islands, the Americas and the Caribbean. Although most ZIKV infections are asymptomatic, cases of neurological manifestations have been described. The aim of the present study was to identify the prevalence of ZIKV infection among the asymptomatic persons in Myanmar in 2018.
METHODS
A total of 284 serum samples from apparently healthy persons were collected from Yangon, Myanmar in 2018. They were analysed for ZIKV infection by immunoglobulin M (IgM) capture enzyme-linked immunosorbent assay (ELISA), IgG indirect ELISA, 50% focus reduction neutralization test, real-time reverse transcription polymerase chain reaction (RT-PCR) and conventional RT-PCR.
RESULTS
Of the 284 apparently healthy persons, 31.3% were positive for the presence of IgM against ZIKV and 94.3% were positive for anti-flavivirus IgG. Among the ZIKV IgM-positive samples, we confirmed ZIKV infection in 15.8% of asymptomatic persons by neutralization test and real-time RT-PCR.
CONCLUSIONS
We conclude that ZIKV infection was increasing among asymptomatic persons in the same area in Myanmar during 2018 compared with 2017. It is highly recommended to strengthen the surveillance system for ZIKV to prevent possible outbreaks.
背景
Zika 病毒（ZIKV）是一种通过蚊子传播的黄病毒。ZIKV 感染的爆发曾发生在非洲、东南亚、太平洋岛屿、美洲和加勒比地区。尽管大多数 ZIKV 感染是无症状的，但已描述了神经系统表现的病例。本研究旨在确定 2018 年缅甸无症状人群中 ZIKV 感染的流行情况。
方法
2018 年从缅甸仰光采集了 284 份来自明显健康人群的血清样本。通过免疫球蛋白 M（IgM）捕获酶联免疫吸附试验（ELISA）、IgG 间接 ELISA、50% 焦点减少中和试验、实时逆转录聚合酶链反应（RT-PCR）和常规 RT-PCR 分析 ZIKV 感染情况。
结果
在 284 名明显健康人群中，31.3% 的人对 ZIKV 的 IgM 呈阳性，94.3% 的人对黄病毒 IgG 呈阳性。在 ZIKV IgM 阳性样本中，通过中和试验和实时 RT-PCR 确认了 15.8%的无症状人群存在 ZIKV 感染。
结论
我们得出结论，与 2017 年相比，2018 年缅甸同一地区无症状人群中的 ZIKV 感染呈上升趋势。强烈建议加强 ZIKV 监测系统，以预防可能的爆发。
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