Meksuriyen D, Cordell G A
Program for Collaborative Research in the Pharmaceutical Sciences, College of Pharmacy, University of Illinois, Chicago 60612.
J Nat Prod. 1988 Sep-Oct;51(5):893-9. doi: 10.1021/np50059a013.
Following studies to define the time course, media, and nutrient parameters for the production of the potent cytotoxic antibiotic staurosporine in Streptomyces staurosporeus, biosynthetic studies of staurosporine with singly- and doubly-labeled radioactive precursors established that either one or two units of tryptophan was incorporated efficiently. From the 13C-nmr spectrum of staurosporine subsequent to stable isotope incorporation experiments, it was established that the aglycone moiety was derived from two units of tryptophan with the carbon skeleton incorporated intact.
在对星孢链霉菌中强效细胞毒性抗生素星形孢菌素生产的时间进程、培养基和营养参数进行定义的研究之后，使用单标记和双标记放射性前体对星形孢菌素进行的生物合成研究表明，一个或两个色氨酸单元能够有效地掺入。通过稳定同位素掺入实验后星形孢菌素的13C核磁共振谱，确定苷元部分源自两个色氨酸单元，且碳骨架完整掺入。
