环状 RNA 表达谱及冠心病的生物信息学分析。
Circular RNA expression profiles and bioinformatic analysis in coronary heart disease.
机构信息
The Key Laboratory of Cardiovascular Remodeling & Function Research, Chinese Ministry of Education, Chinese Ministry of Health & Chinese Academy of Medical Sciences, & The State & Shandong Province Joint Key Laboratory of Translational Cardiovascular Medicine, Qilu Hospital of Shandong University, Jinan 250012, Shandong, PR China.
The Cardiology Department of Yuhuangding Hospital, Qingdao University School of Medicine, Yantai 264000, Shandong, PR China.
出版信息
Epigenomics. 2020 Mar;12(5):439-454. doi: 10.2217/epi-2019-0369. Epub 2020 Feb 11.
We aimed to identify the expression profile and role of circular RNAs (circRNAs) in coronary heart disease (CHD). We performed sequence analysis of circRNAs in peripheral blood mononuclear cells of 70 CHD patients and 30 controls. Eight selected circRNAs were validated using quantitative real-time polymerase chain reaction (qRT-PCR) in human atherosclerotic coronary arteries. In total, 2283 downregulated and 85 upregulated circRNAs were identified in CHD. Parental genes of top 100 dysregulated-circRNAs are related to metabolism and protein modification, and 12 circRNAs might upregulate their CHD-related parental genes through miRNA sponges. Of the eight circRNAs validated in atherosclerotic coronary arteries by qRT-PCR, six were consistent with sequencing results of peripheral blood mononuclear cells. As potential ceRNAs, dysregulated circRNAs may be involved in CHD pathophysiology.
我们旨在确定环状 RNA（circRNA）在冠心病（CHD）中的表达谱和作用。我们对 70 例 CHD 患者和 30 例对照者的外周血单个核细胞中的 circRNA 进行了序列分析。使用定量实时聚合酶链反应（qRT-PCR）在人动脉粥样硬化性冠状动脉中验证了 8 个选定的 circRNA。在 CHD 中总共鉴定出 2283 个下调和 85 个上调的 circRNA。前 100 个失调 circRNA 的亲本基因与代谢和蛋白质修饰有关，并且 12 个 circRNA 可能通过 miRNA 海绵作用上调其与 CHD 相关的亲本基因。通过 qRT-PCR 在动脉粥样硬化性冠状动脉中验证的 8 个 circRNA 中，有 6 个与外周血单个核细胞的测序结果一致。作为潜在的 ceRNA，失调的 circRNA 可能参与 CHD 病理生理学。
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