Cardiovascular Research Center, The First Affiliated Hospital of Xinxiang Medical University, Xinxiang, Henan 453100, P.R. China.
Mol Med Rep. 2019 Apr;19(4):2527-2536. doi: 10.3892/mmr.2019.9905. Epub 2019 Jan 28.
Coronary heart disease (CHD) is a major cause of morbidity and mortality and an important public health problem globally, but the mechanism of CHD is still complex and unclear. The purpose of the current study was to explore the mechanism underlying CHD using high‑throughput technology. The study participants were patients with coronary angiography (CAG)‑proven severity of coronary artery stenosis. Patients were divided into control and test group based on specific inclusion criteria, and data were collected regarding the results of routine inspection and the Gensini score (GS). We explored the mechanism underlying CHD with high‑throughput integration of circular RNA (circRNA)‑microRNA (miRNA) data. Through the expression of circRNA‑miRNA, we discovered a total of 110 circRNAs to be differentially expressed in the two groups. Of these, 73 were upregulated and 37 downregulated in the CHD (fold ≥2.0 and P<0.05). Among 18 miRNAs, 13 were upregulated and 5 were downregulated in the CHD group (fold ≥2.0 and P<0.05). Enrichment analysis showed that circRNAs participate in a variety of disease development processes, biological processes, molecular functions, cellular components, and pathways (P<0.05). The mechanism underlying CHD may be closely related to up‑ or downregulated circRNA and miRNA and co‑expression of circRNA‑miRNA specifically involved regulate multiple pathways and multiple cellular and molecular biological processes.
冠心病（CHD）是发病率和死亡率的主要原因，也是全球重要的公共卫生问题，但 CHD 的发病机制仍较为复杂，尚不明确。本研究旨在利用高通量技术探索 CHD 的发病机制。研究对象为经冠状动脉造影（CAG）证实的冠状动脉狭窄严重程度的患者。根据具体纳入标准，将患者分为对照组和试验组，并收集常规检查结果和 Gensini 评分（GS）的数据。我们通过环状 RNA（circRNA）-微小 RNA（miRNA）高通量整合来探索 CHD 的发病机制。通过 circRNA-miRNA 的表达，我们在两组中总共发现了 110 个差异表达的 circRNA。其中，CHD 组有 73 个上调，37 个下调（倍数≥2.0 和 P<0.05）。在 18 个 miRNA 中，CHD 组有 13 个上调，5 个下调（倍数≥2.0 和 P<0.05）。富集分析表明，circRNA 参与多种疾病的发生发展过程、生物学过程、分子功能、细胞成分和通路（P<0.05）。CHD 的发病机制可能与上调或下调的 circRNA 和 miRNA 密切相关，circRNA-miRNA 的共表达特异性参与调节多个通路和多个细胞及分子生物学过程。
