State Key Laboratory of Biocontrol and Guangdong Key Laboratory of Plant Resources, School of Life Sciences, Sun Yat-Sen University, Guangzhou, 510275, PR China.
CAS Key Laboratory of Biogeography and Bioresource in Arid Land, Xinjiang Institute of Ecology and Geography, Urumqi 830011, PR China.
Int J Syst Evol Microbiol. 2020 Apr;70(4):2298-2304. doi: 10.1099/ijsem.0.004035. Epub 2020 Feb 11.
A novel thermotolerant bacterial strain was isolated from a hot spring in a Tibetan geothermal field. Phylogenetic analysis of the 16S rRNA gene sequence of the novel strain showed that it belongs to a distinct lineage far from any known taxa. The new isolate shared the highest pairwise sequence identity with S-12 (92.8 % similarity) according to the 16S rRNA gene sequences. Cells were Gram-stain-negative, aerobic, rod-shaped and formed white round colonies. The strain grew at the ranges of 28-45 °C (optimum, 37 °C), pH 5.0-7.0 (optimum, pH 6.0) and 0-2 % NaCl. The strain was positive for catalase and oxidase. The major respiratory quinone was ubiquinone Q-10. Polar lipids consisted of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol and phosphatidylcholine. The major fatty acids were summed feature 8 (C ω7 and/or C ω6). The DNA G+C content was 68.3 mol%. Based on these distinguishing properties, this strain is proposed to represent a new species of a new genus gen. nov., sp. nov., within a new family fam. nov. The type strain of the type species of is SYSU G02060 (=KCTC 72351=CGMCC 1.17070).
从西藏地热田温泉中分离到一株新型耐温细菌。新菌株的 16S rRNA 基因序列的系统发育分析表明，它属于一个与任何已知分类群都很远的独特谱系。根据 16S rRNA 基因序列，新分离株与 S-12（92.8%相似性）的序列相似度最高。细胞革兰氏染色阴性，需氧，杆状，形成白色圆形菌落。该菌株在 28-45°C（最佳温度 37°C）、pH 5.0-7.0（最佳 pH 6.0）和 0-2%NaCl 的范围内生长。该菌株过氧化氢酶和氧化酶阳性。主要呼吸醌为泛醌 Q-10。极性脂类由双磷脂酰甘油、磷脂酰乙醇胺、磷脂酰甘油和磷脂酰胆碱组成。主要脂肪酸为 summed feature 8 (C ω7 和/或 C ω6)。DNA G+C 含量为 68.3mol%。基于这些鉴别特性，该菌株被提议代表一个新属的新种，属于一个新科。该新种的模式菌株是 SYSU G02060（=KCTC 72351=CGMCC 1.17070）。
