Huang Zhaobin, Guo Feng, Lai Qiliang, Shao Zongze
Key Laboratory of Marine Genetic Resources, Third Institute of Oceanography, State of Oceanic Administration, Xiamen, PR China.
School of Life Sciences, Xiamen University, Xiamen, PR China.
Int J Syst Evol Microbiol. 2017 Aug;67(8):2527-2531. doi: 10.1099/ijsem.0.001951. Epub 2017 Aug 4.
A Gram-stain-negative, short rod-shaped and non-flagellated bacterium, named strain XMTR2A4T, was isolated from the gut of a marine limpet, Notoacmea schrenckii on intertidal rocks. Colonies were small, light grey and circular. Catalase- and oxidase-positive. Growth was observed at 15 to 37 °C (optimum 28-30 °C), with salinity range from 0.5 to 9 % (optimum 1-2 %), and at pH 6 to 9 (optimum pH 7). The almost full-length 16S rRNA gene of strain XMTR2A4T had the highest sequence similarity of 93.7 % with Mycoplana ramosa DSM 7292T, and of 93.5 , 93.5 and 93.4 % with Chelativorans intermedius CC-MHSW-5T, Ensifer fredii ATCC 35423T and Phyllobacterium myrsinacearum IAM 13584T, respectively. Phylogenetic analysis showed that strain XMTR2A4T formed a tight cluster with a cultured but uncharacterized strain, YP382-1-A, which was deeply separated from the species within the order Rhizobiales in the class Alphaproteobacteria. The predominant fatty acid of strain XMTR2A4T was summed feature 8 (C18 : 1ω7c and/or C18 : 1ω6c; 83.4 %). Ubiquinone-10 (Q-10) was detected as the sole respiratory quinone. The polar lipids were identified as phosphatidylethanolamine, phosphatidylglycerol, phosphatidylcholine, diphosphatidylglycerol, phosphatidylmonomethylethanolamine, an unidentified phospholipid and three unknown lipids. The genome size was about 3.6 Mbp and the G+C content was 61.5 mol%. Combining the results above, it was ascertained that strain XMTR2A4Trepresents a novel species of a new genus in the order Rhizobiales, for which the name Notoacmeibacter marinus gen. nov., sp. nov. is proposed. The type strain of the type species is XMTR2A4T (=MCCC 1A01882T=KCTC 52427T). A novel family in the order Rhizobiales, named Notoacmeibacteraceae fam. nov., is also proposed to accommodate the new genus.
从潮间带岩石上的海洋帽贝（史氏背尖贝）肠道中分离出一株革兰氏染色阴性、短杆状且无鞭毛的细菌，命名为菌株XMTR2A4T。菌落小，呈浅灰色，圆形。过氧化氢酶和氧化酶呈阳性。在15至37°C（最适温度28 - 30°C）、盐度范围为0.5%至9%（最适盐度1 - 2%）以及pH值为6至9（最适pH值7）的条件下可生长。菌株XMTR2A4T的近乎全长16S rRNA基因与分枝状枝动菌DSM 7292T的序列相似性最高，为93.7%，与中间螯合菌CC - MHSW - 5T、费氏中华根瘤菌ATCC 35423T和紫金牛叶杆菌IAM 13584T的序列相似性分别为93.5%、93.5%和93.4%。系统发育分析表明，菌株XMTR2A4T与一株已培养但未鉴定的菌株YP382 - 1 - A形成紧密聚类，该聚类与α - 变形菌纲根瘤菌目中的物种深度分离。菌株XMTR2A4T的主要脂肪酸为总和特征8（C18:1ω7c和/或C18:1ω6c；83.4%）。检测到泛醌 - 10（Q - 10）为唯一的呼吸醌。极性脂质鉴定为磷脂酰乙醇胺、磷脂酰甘油、磷脂酰胆碱、二磷脂酰甘油、磷脂酰单甲基乙醇胺、一种未鉴定的磷脂和三种未知脂质。基因组大小约为3.6 Mbp，G + C含量为61.5 mol%。综合上述结果，确定菌株XMTR2A4T代表根瘤菌目中一个新属的新物种，为此提出新属名海洋史氏背尖菌属（Notoacmeibacter marinus），新种名海洋史氏背尖菌（Notoacmeibacter marinus gen. nov., sp. nov.）。模式种的模式菌株为XMTR2A4T（=MCCC 1A01882T=KCTC 52427T）。还提出了根瘤菌目中一个新科，命名为海洋史氏背尖菌科（Notoacmeibacteraceae fam. nov.），以容纳这个新属。
