属新名，种新名，属新名，种新名，种新名，均为从西南印度洋脊深海水中分离得到的 3 个新成员。
gen. nov., sp. nov., gen. nov., sp. nov. and sp. nov., three novel members isolated from deep-sea water in the southwest Indian ridge.
机构信息
Key Laboratory of Marine Ecosystem Dynamics, Ministry of Natural Resources & Second Institute of Oceanography, Ministry of Natural Resources, Hangzhou 310012, PR China.
School of Oceanography, Shanghai Jiao Tong University, Shanghai 200030, PR China.
出版信息
Int J Syst Evol Microbiol. 2022 Feb;72(2). doi: 10.1099/ijsem.0.005236.
Three Gram-staining-negative, aerobic and rod-shaped strains, designated as T40-1, T40-3 and JL-62, were isolated from the deep-sea water in the southwest Indian ridge. For strain T40-1, growth occurred at 15-37 °C (optimum, 28 °C), pH 6.0-9.0 (optimum, pH 7.5) and in the presence of 0.5-5.0 % NaCl (w/v; optimum, 2.0 %). Strain T40-3 could grow at 15-40 °C (optimum, 28 °C), with 0.5-11.0 % NaCl (optimum, 2.0 %, w/v) at pH 6.0-9.5 (optimum, 8.0). The temperature, pH and salinity ranges for growth of strain JL-62 were 15-40 °C (optimum, 30 °C), pH 5.5-9.0 (optimum, pH 7.5-8.0) and 0.5-9.0 % NaCl (w/v; optimum, 4.0 %). Ubiquinone-10 was the sole ubiquinone in all strains, the major fatty acids (>20 %) were summed feature 8 (C 7 / C 6). The major polar lipids of strains T40-1 and T40-3 were phosphatidylcholine, phosphatidylglycerol, phosphatidylethanolamine and diphosphatidylglycerol. Strain JL-62 contained phosphatidylmonomethylethanolamine, diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol and sulfoquinovosyldiacylglycerol as major polar lipids. Phylogenetic trees based on 16S rRNA gene and core-genomic sequences revealed affiliation of strains T40-1and T40-3 to the family and formed two independent clades from other genera, and those two strains had average nucleotide identities of 62.0-72.0 % to their phylogenetically related species which fell into to the genus boundary range, indicating that they represent two novel genera. While strain JL-62 represents a novel species in the genus belonging to the family , which was supported by overall genomic relatedness index calculations. The DNA G+C contents of strains T40-1, T40-3 and JL-62 were 66.5, 60.1 and 62.1 mol %, respectively. Based on the polyphasic taxonomic data, strains T40-1 (=MCCC M24557=KCTC 82975) and T40-3 (=MCCC 1K05135=KCTC 82976) are classified as representing two novel genera belonging to the family with the names gen. nov., sp. nov. and gen. nov., sp. nov. are proposed, and strain JL-62 (=MCCC M24579=KCTC 82974) is proposed to represent a novel species within the genus with the name sp. nov. is proposed.
三株革兰氏阴性、需氧、杆状的菌株，分别命名为 T40-1、T40-3 和 JL-62，从西南印度洋海水中分离得到。对于菌株 T40-1，生长温度为 15-37°C（最佳温度为 28°C），pH 值为 6.0-9.0（最佳 pH 值为 7.5），盐度为 0.5-5.0%NaCl（w/v；最佳盐度为 2.0%）。菌株 T40-3 可在 15-40°C（最佳温度为 28°C）下生长，盐度为 0.5-11.0%NaCl（最佳盐度为 2.0%，w/v），pH 值为 6.0-9.5（最佳 pH 值为 8.0）。菌株 JL-62 的生长温度、pH 值和盐度范围分别为 15-40°C（最佳温度为 30°C）、pH 值为 5.5-9.0（最佳 pH 值为 7.5-8.0）和 0.5-9.0%NaCl（w/v；最佳盐度为 4.0%）。所有菌株均以泛醌-10 作为唯一的泛醌，主要脂肪酸（>20%）为 summed feature 8（C 7 / C 6）。菌株 T40-1 和 T40-3 的主要极性脂为磷脂酰胆碱、磷脂酰甘油、磷脂酰乙醇胺和双磷脂酰甘油。菌株 JL-62 含有磷脂单甲基乙醇胺、双磷脂酰甘油、磷脂酰乙醇胺、磷脂酰甘油和磺基奎诺二酰甘油作为主要极性脂。基于 16S rRNA 基因和核心基因组序列构建的系统发育树表明，菌株 T40-1 和 T40-3 属于 科，与其他属的菌株形成两个独立的分支，这两个菌株与亲缘关系最近的种的平均核苷酸同一性为 62.0-72.0%，处于属的边界范围内，表明它们代表两个新的属。而菌株 JL-62 则代表了 属中的一个新种，属于 科，这一点得到了全基因组相关指数计算的支持。菌株 T40-1、T40-3 和 JL-62 的 DNA G+C 含量分别为 66.5、60.1 和 62.1mol%。基于多相分类学数据，菌株 T40-1（=MCCC M24557=KCTC 82975）和 T40-3（=MCCC 1K05135=KCTC 82976）被分类为代表两个新属，属于 科，分别命名为 属，新种。和 属，新种。提议，菌株 JL-62（=MCCC M24579=KCTC 82974）被提议为 属内的一个新种，命名为 种，新种。
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