Department of Neurobiology, Howard Hughes Medical Institute, Harvard Medical School, Boston, United States.
Elife. 2020 Feb 11;9:e51271. doi: 10.7554/eLife.51271.
The lateral habenula (LHb) is an epithalamic brain structure critical for processing and adapting to negative action outcomes. However, despite the importance of LHb to behavior and the clear anatomical and molecular diversity of LHb neurons, the neuron types of the habenula remain unknown. Here, we use high-throughput single-cell transcriptional profiling, monosynaptic retrograde tracing, and multiplexed FISH to characterize the cells of the mouse habenula. We find five subtypes of neurons in the medial habenula (MHb) that are organized into anatomical subregions. In the LHb, we describe four neuronal subtypes and show that they differentially target dopaminergic and GABAergic cells in the ventral tegmental area (VTA). These data provide a valuable resource for future study of habenular function and dysfunction and demonstrate neuronal subtype specificity in the LHb-VTA circuit.
外侧缰核（LHb）是一种脑内结构，对于处理和适应负面行为结果至关重要。然而，尽管 LHb 对行为很重要，且 LHb 神经元具有明显的解剖学和分子多样性，但缰核的神经元类型仍不清楚。在这里，我们使用高通量单细胞转录组学 profiling、单突触逆行示踪和多重荧光原位杂交（FISH）来描述小鼠缰核的细胞。我们发现内侧缰核（MHb）中有 5 种神经元亚型，它们被组织成解剖亚区。在 LHb 中，我们描述了 4 种神经元亚型，并表明它们在多巴胺能和 GABA 能细胞中具有不同的靶向性，这些细胞位于腹侧被盖区（VTA）中。这些数据为缰核功能和功能障碍的未来研究提供了有价值的资源，并证明了 LHb-VTA 回路中的神经元亚型特异性。
