Adolfo Lutz Institute, São Paulo, SP, Brazil.
Department of Zoology, University of Oxford, United Kingdom.
Acta Trop. 2020 May;205:105390. doi: 10.1016/j.actatropica.2020.105390. Epub 2020 Feb 7.
The southeastern region of Brazil has recently experienced the largest yellow fever disease outbreak in decades. Since July 2016 epizootic events were reported in São Paulo state's north region, where 787 Culicidae were captured as part of public health surveillance efforts and tested using real-time quantitative PCR. One Aedes scapularis pool collected in November 2016 in an agriculture area in Urupês city tested positive for YFV-RNA. Using a validated multiplex PCR approach we were able to recover a complete virus genome sequence from this pool. Phylogenetic analysis of the novel strain and publicly available data indicates that the belongs to the South American genotype 1 clade circulating in Sao Paulo state and is basal to the recent outbreak clade in southeast Brazil. Our findings highlight the need of additional studies, including vector competence studies, to disentangle the role of Aedes scapularis in yellow fever transmission in the Americas.
巴西东南部地区最近经历了几十年来最大规模的黄热病疫情爆发。自 2016 年 7 月以来，在圣保罗州北部地区报告了疫情事件，在那里作为公共卫生监测工作的一部分捕获了 787 只库蚊，并使用实时定量 PCR 进行了测试。2016 年 11 月在乌鲁普斯市一个农业区采集的一只埃及伊蚊蚊群检测出黄热病毒 RNA 阳性。使用经过验证的多重 PCR 方法，我们能够从该蚊群中恢复出完整的病毒基因组序列。对新型菌株和公开数据的系统发育分析表明，该菌株属于在圣保罗州流行的南美基因型 1 分支，并且是巴西东南部最近爆发分支的基础。我们的研究结果强调需要开展更多的研究，包括媒介效能研究，以阐明埃及伊蚊在美洲黄热病传播中的作用。
