Department of Chemical Engineering, Faculty of Science and Engineering, University of Nottingham Malaysia, 43500 Selangor Darul Ehsan, Malaysia.
School of Mathematical Sciences, Faculty of Science and Engineering, University of Nottingham Malaysia, 43500 Selangor Darul Ehsan, Malaysia.
Bioresour Technol. 2020 May;303:122931. doi: 10.1016/j.biortech.2020.122931. Epub 2020 Jan 29.
The aim of this work was to study the ultrasonication-assisted Liquid Tri-phasic Flotation (LTF) System to obtain lipid and protein from microalgae Chlorella sorokiniana in a single step as a novel process. In the current study, biorefinery of Chlorella sorokiniana was performed using LTF system in a single step. The highest protein recovery of 97.43 ± 1.67% and lipid recovery of 69.50 ± 0.54% were obtained. The corresponding parameters were microalgae biomass loading of 0.5 w/v%, ammonium sulphate concentration of 40 w/v%, volume ratio of 1:1.5 (salt:alcohol), ultrasonication pulse mode of 20 s ON/20 s OFF at 20% amplitude for 5 mins, flotation air flowrate of 100 mL/min. Additionally, recycling of alcohol phase to study the circular nature of proposed biorefinery was investigated. The proposed LTF system for extraction of proteins and lipid reduces the number of operation units required in this biorefinery approach.
本工作旨在研究超声辅助三相液-液浮选（LTF）系统，以一步法从微藻衣藻中同时获得脂质和蛋白质，这是一种新颖的工艺。在本研究中，采用 LTF 系统一步法对衣藻进行了生物炼制。获得了最高的蛋白质回收率 97.43±1.67%和脂质回收率 69.50±0.54%。相应的参数为微藻生物量装载量 0.5 w/v%、硫酸铵浓度 40 w/v%、体积比 1:1.5（盐：醇）、超声脉冲模式 20 s ON/20 s OFF，幅度为 20%，持续 5 分钟，浮选空气流速为 100 mL/min。此外，还研究了回收醇相以研究所提出的生物炼制的循环性质。所提出的用于提取蛋白质和脂质的 LTF 系统减少了这种生物炼制方法所需的操作单元数量。
