Department of Chemical and Environmental Engineering, Faculty of Science and Engineering, University of Nottingham Malaysia, 43500 Semenyih, Selangor Darul Ehsan, Malaysia.
School of Energy and Chemical Engineering, Xiamen University Malaysia, Jalan Sunsuria, Bandar Sunsuria, 43900 Sepang, Selangor Darul Ehsan, Malaysia; College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, Fujian, China.
Bioresour Technol. 2021 Aug;333:125075. doi: 10.1016/j.biortech.2021.125075. Epub 2021 Mar 31.
Microalgae are potential sustainable renewable sources of energy but are highly underutilized due to the expensive and time-consuming downstream processing. This study aims at curbing these obstacles by extracting multiple components with a single processing unit. In this work, an ultrasound-assisted liquid triphasic flotation system was incorporated to extract proteins, lipids, and carbohydrates by phase separation. The parameters involved were optimized and the final recovery efficiency of proteins, lipids, and carbohydrates was determined. A control run involving conventional three-phase partitioning and a 15-fold scale-up system with the recycling of phase components were also performed. Gas Chromatograph and Fourier Transform Infrared spectroscopy were used to examine the potential of extracted products as a source of biofuel. This biorefinery approach is crucial in commercializing microalgae for biodiesel and bioethanol generation with a side product of purified proteins as feed.
微藻是有潜力的可持续可再生能源，但由于下游处理过程昂贵且耗时，因此尚未得到充分利用。本研究旨在通过单一处理单元提取多种成分来克服这些障碍。在这项工作中，采用超声辅助液三相浮选系统通过相分离提取蛋白质、脂质和碳水化合物。优化了所涉及的参数，并确定了蛋白质、脂质和碳水化合物的最终回收效率。还进行了涉及常规三相分离的对照运行和 15 倍放大系统以及相成分的循环回收。气相色谱和傅里叶变换红外光谱用于检查提取产物作为生物燃料来源的潜力。这种生物炼制方法对于将微藻商业化用于生物柴油和生物乙醇生产以及作为饲料的纯化蛋白质的副产品至关重要。
