类风湿关节炎大鼠模型脾中 CD4 T 细胞的研究及ω-3 可能的治疗作用。组织学和免疫荧光研究。
A highlight on CD4 T-cells in the spleen in a rat model of rheumatoid arthritis and possible therapeutic effect of omega-3. Histological and Immunofluorescence study.
机构信息
Department of Histology and Cell Biology, Faculty of Medicine, Ain Shams University, Cairo, Egypt.
出版信息
Int Immunopharmacol. 2020 Apr;81:106283. doi: 10.1016/j.intimp.2020.106283. Epub 2020 Feb 8.
BACKGROUND
Rheumatoid arthritis (RA), a primary chronic articular disease with wide range of extra-articular and systemic effects. The spleen is one of the most affected organs in RA. CD4 T cells play an important role in initiation, maintenance and control of the disease.
AIM OF THE WORK
This work was designed to study the histological changes occurring in the spleen in a rat model of RA and to assess the effect of treatment with omega-3 alone, with special refer to the role of CD4 T-cells.
MATERIALS AND METHODS
Thirty male albino rats were divided into four groups; control group, early and progressive RA groups and omega-3 treated group. RA was induced in rats of groups II, III and IV by a single subcutaneous injection of complete Freund's adjuvant (CFA). Samples were taken after two and four weeks of the CFA injection (in early and progressive RA groups respectively). Treatment with omega-3 (300 mg/kg/day in a single, daily oral dose) started two weeks after CFA injection in rats of group IV and continued for another two weeks. Spleen specimens were collected at the appropriate times and processed to obtain paraffin blocks. Sections were then stained for histological and immunofluorescence studies.
RESULTS
Both, early and progressive RA induced noticeable structural changes in the spleen. Thickened capsule and trabeculae and marked congestion of the blood sinusoids of the red pulp were evident. Expansion of the white pulp and areas of mononuclear cellular infiltration were seen, especially in progressive RA. Affection of blood vessel walls was also noticed. Immunofluorescence study showed extensive expression of Anti-CD4 Monoclonal Antibodies especially in progressive RA. Treatment with omega-3 significantly improved the structure of the spleen as detected by both histological and immunofluorescence studies.
CONCLUSION
Omega-3 treatment ameliorated the structural damage of the spleen caused by experimental induction of RA.
背景
类风湿关节炎（RA）是一种主要的慢性关节疾病，具有广泛的关节外和全身影响。脾脏是 RA 最受影响的器官之一。CD4 T 细胞在疾病的启动、维持和控制中发挥重要作用。
目的
本研究旨在研究 RA 大鼠模型中脾脏的组织学变化，并评估单独使用 ω-3 治疗的效果，特别关注 CD4 T 细胞的作用。
材料和方法
将 30 只雄性白化大鼠分为四组：对照组、早期和进展性 RA 组和 ω-3 治疗组。通过单次皮下注射完全弗氏佐剂（CFA）诱导 II、III 和 IV 组大鼠的 RA。在 CFA 注射后两周（分别在早期和进展性 RA 组）和四周（在进展性 RA 组）采集样本。在 IV 组大鼠 CFA 注射后两周开始给予 ω-3（每日 300mg/kg，单次口服剂量）治疗，并持续两周。在适当的时间采集脾脏标本并进行石蜡包埋。然后对切片进行组织学和免疫荧光研究。
结果
早期和进展性 RA 均引起脾脏明显的结构改变。红髓的包膜和小梁增厚，血窦明显充血。白髓扩张和单核细胞浸润区域可见，特别是在进展性 RA 中。血管壁也受到影响。免疫荧光研究显示，特别是在进展性 RA 中，广泛表达抗 CD4 单克隆抗体。ω-3 治疗显著改善了实验诱导 RA 后脾脏的结构。
结论
ω-3 治疗改善了实验诱导 RA 引起的脾脏结构损伤。
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