Mateus Jose, Gómez Natalia, Herrera-Sepúlveda María Teresa, Hidalgo Marylin, Pérez-Torres Jairo, Cuervo Claudia
Facultad de Ciencias, Pontificia Universidad Javeriana, Bogotá, Colombia.
Unidad de Ecología y Sistemática (UNESIS). Laboratorio de Ecología Funciona, Facultad de Ciencias, Pontificia Universidad Javeriana, Bogotá, Colombia.
J Infect Dev Ctries. 2019 Apr 30;13(4):278-283. doi: 10.3855/jidc.10642.
Bats have become an epidemiologically significant source of pathogenic microorganisms, such as leptospires, the causative agents of leptospirosis. However, little information exists about bats and their potential role as a reservoir of pathogenic Leptospira spp. in Colombia. The aim of this study was to evaluate the presence of pathogenic Leptospira spp. in the kidneys of bats from the Caribbean region of Colombia deposited in the collection of mammals of the Museo Javeriano de Historia Natural (MPUJ-MAMM).
DNA was extracted from twenty-six kidney samples from a total of 13 species of bats captured in Colombia. First, 16S ribosomal RNA conventional PCR was performed to detect the presence of Leptospira spp. Then, in samples that tested positive, LipL32 PCR was performed to detect pathogenic Leptospira spp. by sequencing and phylogenetic analysis.
The presence of Leptospira spp. was observed in 7/26 (26.9%) bats from the following 6 species: Carollia perspicillata, Glossophaga soricina, Dermanura phaeotis, Uroderma bilobatum, Desmodus rotundus, and Lophostoma silvicolum, and pathogenic Leptospira spp. were detected in 4/26 samples (15.4%).
This study suggests that bats present in the Caribbean region of Colombia could be potential reservoirs of pathogenic Leptospira spp.
蝙蝠已成为致病性微生物在流行病学上的重要来源，例如钩端螺旋体，它是钩端螺旋体病的病原体。然而，关于哥伦比亚蝙蝠及其作为致病性钩端螺旋体属储存宿主的潜在作用的信息却很少。本研究的目的是评估保存在哈韦里亚纳自然历史博物馆哺乳动物标本馆（MPUJ-MAMM）中的哥伦比亚加勒比地区蝙蝠肾脏中致病性钩端螺旋体属的存在情况。
从在哥伦比亚捕获的总共13种蝙蝠的26个肾脏样本中提取DNA。首先，进行16S核糖体RNA常规PCR以检测钩端螺旋体属的存在。然后，在检测呈阳性的样本中，进行LipL32 PCR，通过测序和系统发育分析来检测致病性钩端螺旋体属。
在来自以下6种蝙蝠的7/26（26.9%）中观察到钩端螺旋体属的存在：秘鲁叶鼻蝠、食果长舌蝠、棕蝠、双色凹脸蝠、普通吸血蝠和森林冠蝠，并且在4/26个样本（15.4%）中检测到致病性钩端螺旋体属。
本研究表明，哥伦比亚加勒比地区的蝙蝠可能是致病性钩端螺旋体属的潜在储存宿主。
