State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, China.
Department of Chemistry, Hong Kong Baptist University, Kowloon Tong, Hong Kong, China.
J Phys Chem B. 2020 Mar 5;124(9):1592-1601. doi: 10.1021/acs.jpcb.9b09741. Epub 2020 Feb 25.
DNA hairpins are widely used to synthesize silver nanoclusters (AgNCs) with excellent optical properties due to their specific secondary structure. Hairpin-AgNCs have been popularly employed for sensoring applications, while no systematic study has been done about the effect of stem sequence on the fluorescence property of hairpin-AgNCs. In this presented work, the synthesizing conditions of hairpin-AgNCs were fully examined first. Then, the effect of percentage content and distribution of GC base pairs as well as stem length on the fluorescence property of hairpin-AgNCs were studied. Intriguing phenomena were observed and basic conclusions were drawn, which would be helpful to understand the hairpin-AgNCs comprehensively and instructional for the applications using hairpin-AgNC probes.
发夹型 DNA 因其特定的二级结构被广泛用于合成具有优异光学性能的银纳米簇（AgNCs）。发夹型 AgNCs 已被广泛应用于传感应用，而对于茎序列对发夹型 AgNCs 荧光性质的影响，尚无系统的研究。在本工作中，首先充分考察了发夹型 AgNCs 的合成条件。然后，研究了 GC 碱基对的百分含量和分布以及茎长对发夹型 AgNCs 荧光性质的影响。观察到了有趣的现象，并得出了基本结论，这有助于全面理解发夹型 AgNCs，并为使用发夹型 AgNC 探针的应用提供指导。
