Department of Analytical Chemistry, Jilin Institute of Chemical Technology, 45 Chengde Street, Jilin, 132022, People's Republic of China.
Anal Sci. 2022 Jan;38(1):131-136. doi: 10.2116/analsci.21P146. Epub 2022 Feb 28.
We have developed a type of low-cost, label-free silver nanocluster molecular beacon-like fluorescence sensor with a DNA template. To detect target DNA with this probe, we use a hairpin DNA sequence based on a "turn-on" strategy. The transformation of hairpin DNA would visibly influence the formation of Ag nanoclusters, such that the stronger fluorescence will be measured with the solution containing target nucleic acids than that without targets nucleic acids. There is a good liner relationship between the fluorescence and the target DNA concentrations, ranging from 1 to 750 nmol L. Importantly, the detection sensing platform allows down to 1 nmol L, which is much lower than other studies.
我们开发了一种低成本、无标记的银纳米簇分子信标样荧光传感器，其具有 DNA 模板。为了用该探针检测靶 DNA，我们使用基于“开启”策略的发夹 DNA 序列。发夹 DNA 的构象变化会明显影响银纳米簇的形成，使得含有靶核酸的溶液的荧光强度明显强于不含靶核酸的溶液。荧光与靶 DNA 浓度之间存在良好的线性关系，范围为 1 至 750 nmol L。重要的是，检测传感平台的检测下限低至 1 nmol L，远低于其他研究。
