College of Resources and Environmental Sciences/ Jiangsu Provincial Key Laboratory of Marine Biology, Nanjing Agricultural University, Nanjing 210095, China.
College of Resources and Environmental Sciences/ Jiangsu Provincial Key Laboratory of Marine Biology, Nanjing Agricultural University, Nanjing 210095, China.
Gene. 2020 May 15;738:144460. doi: 10.1016/j.gene.2020.144460. Epub 2020 Feb 8.
MicroRNA390 (miR390), an ancient and highly conserved miRNA family in land plants, plays multiple roles in plant growth, development and stress responses. In this study, we isolated and identified MIR390, miR390, TAS3a/b/c, tasiARF-1/2/3 (trans-acting small interfering RNAs influencing Auxin Response Factors) and ARF2/3/4 in Jerusalem artichoke (Helianthus tuberosus L.). Treatment with 100 mM NaCl induced expression of miR390, increased cleavage of TAS3, produced high levels of tasiARFs, and subsequently enhanced cleavage of ARF3/4, which was most likely associated with salt tolerance of the plants. In contrast, treatment with 300 mM NaCl inhibited expression of miR390, attenuated cleavage of TAS3, produced a small amount of tasiARFs, and reduced cleavage of ARF3/4. We proposed that ARF2, one of the targets of tasiARFs, induced under salinity was likely to play an active role in salt tolerance of Jerusalem artichoke. The study of the miR390-TAS3-ARF model in Jerusalem artichoke may broaden our understanding of salt tolerance mechanisms, and provides a theoretical support for further genetic identification and breeding crops with increased tolerance to salt stress.
微 RNA390（miR390）是陆地植物中古老且高度保守的 miRNA 家族之一，在植物生长、发育和应激反应中发挥多种作用。在这项研究中，我们从菊芋（Helianthus tuberosus L.）中分离并鉴定了 MIR390、miR390、TAS3a/b/c、tasiARF-1/2/3（影响生长素反应因子的反式作用小干扰 RNA）和 ARF2/3/4。用 100mM NaCl 处理会诱导 miR390 的表达，增加 TAS3 的切割，产生高水平的 tasiARFs，随后增强 ARF3/4 的切割，这很可能与植物的耐盐性有关。相比之下，用 300mM NaCl 处理会抑制 miR390 的表达，减弱 TAS3 的切割，产生少量的 tasiARFs，并减少 ARF3/4 的切割。我们提出，tasiARFs 的靶标之一 ARF2 在盐胁迫下诱导表达，可能在菊芋的耐盐性中发挥积极作用。对菊芋 miR390-TAS3-ARF 模型的研究可能拓宽我们对耐盐机制的理解，并为进一步遗传鉴定和培育耐盐作物提供理论支持。
