Zeng Yanluo, Guo Tianbin, Feng Liping, Yin Zhuoda, Luo Hongli, Yin Hongyan
School of Tropical Agriculture and Forestry, Hainan University, Haikou, Hainan, China.
School of Breeding and Multiplication, Hainan University, Haikou, Hainan, China.
Plant Methods. 2024 Dec 5;20(1):182. doi: 10.1186/s13007-024-01301-4.
In recent years, long non-coding RNAs (lncRNAs) and microRNAs (miRNAs) have emerged as critical regulators in plant biology, governing complex gene regulatory networks. In the context of disease resistance in Hevea brasiliensis, the rubber tree, significant progress has been made in understanding its response to anthracnose disease, a serious threat posed by fungal pathogens impacting global rubber tree cultivation and latex quality. While advances have been achieved in unraveling the genetic and molecular foundations underlying anthracnose resistance, gaps persist in comprehending the regulatory roles of lncRNAs and miRNAs under such stress conditions. The specific contributions of these non-coding RNAs in orchestrating molecular responses against anthracnose in H. brasiliensis remain unclear, necessitating further exploration to uncover strategies that increase disease resistance. Here, we integrate lncRNA sequencing, miRNA sequencing, and degradome sequencing to decipher the regulatory landscape of lncRNAs and miRNAs in H. brasiliensis under anthracnose stress. We investigated the genomic and regulatory profiles of differentially expressed lncRNAs (DE-lncRNAs) and constructed a competitive endogenous RNA (ceRNA) regulatory network in response to pathogenic infection. Additionally, we elucidated the functional roles of HblncRNA29219 and its antisense hbr-miR482a, as well as the miR390-TAS3-ARF pathway, in enhancing anthracnose resistance. These findings provide valuable insights into plant-microbe interactions and hold promising implications for advancing agricultural crop protection strategies. This comprehensive analysis sheds light on non-coding RNA-mediated regulatory mechanisms in H. brasiliensis under pathogen stress, establishing a foundation for innovative approaches aimed at enhancing crop resilience and sustainability in agriculture.
近年来，长链非编码RNA（lncRNAs）和微小RNA（miRNAs）已成为植物生物学中的关键调节因子，调控着复杂的基因调控网络。在巴西橡胶树的抗病性方面，人们在了解其对炭疽病的反应方面取得了重大进展，炭疽病是由真菌病原体构成的严重威胁，影响着全球橡胶树的种植和乳胶质量。虽然在揭示炭疽病抗性的遗传和分子基础方面已取得进展，但在理解lncRNAs和miRNAs在这种胁迫条件下的调控作用方面仍存在差距。这些非编码RNA在协调巴西橡胶树对炭疽病的分子反应中的具体作用仍不清楚，需要进一步探索以发现提高抗病性的策略。在这里，我们整合了lncRNA测序、miRNA测序和降解组测序，以破译巴西橡胶树在炭疽病胁迫下lncRNAs和miRNAs的调控格局。我们研究了差异表达lncRNAs（DE-lncRNAs）的基因组和调控图谱，并构建了一个响应病原体感染的竞争性内源RNA（ceRNA）调控网络。此外，我们阐明了HblncRNA29219及其反义hbr-miR482a以及miR390-TAS3-ARF途径在增强炭疽病抗性中的功能作用。这些发现为植物-微生物相互作用提供了有价值的见解，并对推进农作物保护策略具有广阔的意义。这一全面分析揭示了病原体胁迫下巴西橡胶树中由非编码RNA介导的调控机制，为旨在提高农业作物恢复力和可持续性的创新方法奠定了基础。
