白细胞介素-18 基因座的遗传变异与汉族人群中依赖小麦的运动诱发过敏反应有关。

Genetic variation at the interleukin-18 locus is associated with wheat-dependent exercise-induced anaphylaxis in the Han Chinese population.

机构信息

Department of Allergy, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China; Beijing Key Laboratory of Precision Medicine for Diagnosis and Treatment of Allergic Diseases, Beijing, China.

出版信息

Gene. 2020 May 5;737:144462. doi: 10.1016/j.gene.2020.144462. Epub 2020 Feb 8.

DOI:10.1016/j.gene.2020.144462

PMID:32045661

Abstract

OBJECTIVE

To investigate the association of single nucleotide polymorphisms (SNPs) at the interleukin (IL)-18 locus with wheat-dependent exercise-induced anaphylaxis (WDEIA) in the Han Chinese population.

METHOD

130 patients with WDEIA and 600 healthy subjects were recruited for this study. Three tag SNPs (rs360729, rs360717 and rs1946518) were selected and genotyped, and total IgE and specific IgE levels were measured using the ImmunoCAP system.

RESULTS

After Bonferroni correction, the frequency of the G-allele in rs1946518 in the WDEIA group was significantly higher than that in control group (P = 0.0015). Genotypic distributions of rs1946518 significantly differed between WDEIA and control groups, and compared with the TT genotype, the homozygote GG genotype was associated with a higher risk of WDEIA (P = 0.0031). At position rs1946518, the log-transformed total IgE values were significantly higher in the group with the heterozygous GT genotype than in TT genotype group (P = 0.0024). Haplotype AGG formed by three SNPs alleles occurred significantly more frequently in WDEIA group than in control group (P = 0.003).

CONCLUSION

The minor allele G at position rs1946518 might serve as a marker for the risk of WDEIA.

摘要

目的

探讨白细胞介素（IL）-18 基因座单核苷酸多态性（SNP）与汉族人群小麦依赖运动诱发的过敏（WDEIA）的相关性。

方法

本研究纳入了 130 例 WDEIA 患者和 600 名健康对照者。选择了 3 个标签 SNP（rs360729、rs360717 和 rs1946518）并进行基因分型，使用 ImmunoCAP 系统测量总 IgE 和特异性 IgE 水平。

结果

经过 Bonferroni 校正后，WDEIA 组 rs1946518 的 G 等位基因频率明显高于对照组（P=0.0015）。rs1946518 的基因型分布在 WDEIA 组和对照组之间存在显著差异，与 TT 基因型相比，GG 纯合基因型与 WDEIA 的风险增加相关（P=0.0031）。在 rs1946518 位置，杂合 GT 基因型的总 IgE 值明显高于 TT 基因型组（P=0.0024）。三个 SNP 等位基因组成的单倍型 AGG 在 WDEIA 组中比对照组更频繁出现（P=0.003）。