文冠果壳中的化学成分。
Chemical constituents from shells of Xanthoceras sorbifolium.
机构信息
School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, Shenyang, 110016, Liaoning, China.
School of Life Science and Biopharmaceutics, Shenyang Pharmaceutical University, 103 Wenhua Road, Shenyang, China.
出版信息
Phytochemistry. 2020 Apr;172:112288. doi: 10.1016/j.phytochem.2020.112288. Epub 2020 Feb 8.
Three undescribed triterpenes and four previously unreported saponins, along with two known ones, were isolated from shells of Xanthoceras sorbifolium (Sapindaceae). Their structures were elucidated by the interpretation of 1D and 2D NMR data. The nitric oxide (NO) assay revealed that 28-O-isobutyryl-21-O-angeloyl-R-barrigenol and 3-O-β-D-6-O-methylglucuronopyranosyl-21,22-di-O-angeloyl-R-barrigenol possessed stronger inhibitory effects on LPS-induced NO overproduction (IC = 18.5 ± 1.2 and 28.2 ± 1.8 μM, respectively) than the positive drug minocycline (IC = 30.1 ± 1.3 μM) in activated BV2 cells. Western blot, RT-qPCR, and docking experiments further validated that the regulation of iNOS and IL-1β expressions was involved in the anti-neuroinflammatory effects of these two compounds.
从文冠果（无患子科）的壳中分离得到三种未被描述的三萜和四种以前未报道过的皂苷，以及两种已知的化合物。通过对 1D 和 2D NMR 数据的解释，确定了它们的结构。一氧化氮（NO）测定表明，28-O-异丁酰基-21-O-当归酰基-R-巴尔里根醇和 3-O-β-D-6-O-甲基葡萄糖醛酸吡喃糖苷基-21,22-二-O-当归酰基-R-巴尔里根醇对 LPS 诱导的 NO 过量产生具有更强的抑制作用（IC=18.5±1.2 和 28.2±1.8μM，分别）比阳性药物米诺环素（IC=30.1±1.3μM）在激活的 BV2 细胞中。Western blot、RT-qPCR 和对接实验进一步验证了这两种化合物的抗炎作用涉及对 iNOS 和 IL-1β 表达的调节。
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