Appel A M, Hopson C N, Herman J H
Department of Internal Medicine, University of Cincinnati College of Medicine, OH.
J Rheumatol. 1988 Oct;15(10):1515-24.
Conditioned media derived from explant cultures of human osteoarthritic synovial tissue have been shown to contain preformed and newly synthesized factors of variable molecular weight which are capable on a concentration dependent basis of modulating cartilage proteoglycan metabolism. Anabolic inhibitory and stimulatory activity often appeared to coexist, a reversible down-regulation usually dominating in unfractionated preparations. The size of newly synthesized proteoglycan aggregates and monomers and the length of glycosaminoglycan chains produced in the presence of conditioned media were normal. The pattern of anabolic response did not necessarily correlate with the presence of catabolic inducing activity.
源自人骨关节炎滑膜组织外植体培养的条件培养基已被证明含有分子量可变的预先形成的和新合成的因子，这些因子能够在浓度依赖性基础上调节软骨蛋白聚糖代谢。合成代谢抑制和刺激活性似乎常常共存，在未分级的制剂中通常以可逆的下调为主。在条件培养基存在下产生的新合成的蛋白聚糖聚集体和单体的大小以及糖胺聚糖链的长度是正常的。合成代谢反应模式不一定与分解代谢诱导活性的存在相关。
