Carney S L, Billingham M E, Muir H, Sandy J D
J Orthop Res. 1984;2(3):201-6. doi: 10.1002/jor.1100020301.
The turnover of proteoglycans (assessed by the release into the medium of newly synthesised [35S]-proteoglycan) in explant cultures of articular cartilage from various anatomical sites of the knee joints (stifle) of mature beagles with experimental osteoarthritis has been studied with the following findings: (a) The proportion of newly synthesised proteoglycans released from cartilage explants maintained in vitro was generally increased for cartilage from operated compared with nonoperated control joints. (b) At 3 weeks after surgery there was a significant increase in the release of [35S]-proteoglycans from explants of the lateral and medial tibial plateaux of operated joints compared with sham-operated joints but not from other sites. On the other hand, when this comparison was made at 3 to 6 months after surgery, significant increases in the release of [35S]-proteoglycans were observed from cartilage of all anatomical areas except the patellar groove. (c) The release of [35S]-proteoglycan from cartilage explant cultures was dependent on live chondrocytes, since freeze-thawing the tissue immediately after labelling markedly reduced the release from both normal and osteoarthritic cartilage.
对患有实验性骨关节炎的成年比格犬膝关节（ stifle ）不同解剖部位的关节软骨外植体培养物中蛋白聚糖的周转率（通过新合成的[35S] - 蛋白聚糖释放到培养基中来评估）进行了研究，结果如下：(a) 与未手术的对照关节相比，体外培养的手术关节软骨释放的新合成蛋白聚糖比例通常增加。(b) 术后3周，与假手术关节相比，手术关节外侧和内侧胫骨平台外植体释放的[35S] - 蛋白聚糖显著增加，但其他部位未增加。另一方面，术后3至6个月进行此比较时，除髌槽外，所有解剖区域的软骨释放的[35S] - 蛋白聚糖均显著增加。(c) 软骨外植体培养物中[35S] - 蛋白聚糖的释放依赖于活的软骨细胞，因为标记后立即对组织进行冻融显著降低了正常和骨关节炎软骨的释放。
