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岩藻聚糖硫酸酯抑制骨肉瘤细胞的作用具有种属和分子量依赖性。

Fucoidan Inhibition of Osteosarcoma Cells Is Species and Molecular Weight Dependent.

机构信息

Department of Materials Science and Engineering, University of Sheffield, Sheffield S1 3JD, UK.

INSIGNEO Institute for in Silico Medicine, University of Sheffield, Sheffield S1 3JD, UK.

出版信息

Mar Drugs. 2020 Feb 8;18(2):104. doi: 10.3390/md18020104.

DOI:10.3390/md18020104
PMID:32046368
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7074035/
Abstract

Fucoidan is a brown algae-derived polysaccharide having several biomedical applications. This study simultaneously compares the anti-cancer activities of crude fucoidans from and , and effects of low (LMW, 10-50 kDa), medium (MMW, 50-100 kDa) and high (HMW, >100 kDa) molecular weight fractions of fucoidan against osteosarcoma cells. Glucose, fucose and acid levels were lower and sulphation was higher in crude fucoidan compared to crude fucoidan. MMW had the highest levels of sugars, acids and sulphation among molecular weight fractions. There was a dose-dependent drop in focal adhesion formation and proliferation of cells for all fucoidan-types, but fucoidan and HMW had the strongest effects. G1-phase arrest was induced by fucoidan, MMW and HMW, however fucoidan treatment also caused accumulation in the sub-G1-phase. Mitochondrial damage occurred for all fucoidan-types, however fucoidan led to mitochondrial fragmentation. Annexin V/PI, TUNEL and cytochrome c staining confirmed stress-induced apoptosis-like cell death for fucoidan and features of stress-induced necrosis-like cell death for fucoidans. There was also variation in penetrability of different fucoidans inside the cell. These differences in anti-cancer activity of fucoidans are applicable for osteosarcoma treatment.

摘要

岩藻聚糖是一种从褐藻中提取的多糖，具有多种生物医学应用。本研究同时比较了 和 褐藻粗岩藻聚糖的抗癌活性，以及 褐藻低分子量（LMW，10-50 kDa）、中分子量（MMW，50-100 kDa）和高分子量（HMW，>100 kDa）级分对骨肉瘤细胞的影响。与 褐藻粗岩藻聚糖相比， 褐藻粗岩藻聚糖的葡萄糖、岩藻糖和酸水平较低，硫酸化程度较高。在分子量级分中，MMW 具有最高水平的糖、酸和硫酸化。所有岩藻聚糖类型都导致粘着斑形成和细胞增殖呈剂量依赖性下降，但 褐藻聚糖和 HMW 具有最强的作用。 褐藻聚糖、MMW 和 HMW 诱导 G1 期阻滞，但 褐藻聚糖处理也导致亚 G1 期积累。所有岩藻聚糖类型都导致线粒体损伤，但 褐藻聚糖导致线粒体碎片化。Annexin V/PI、TUNEL 和细胞色素 c 染色证实 褐藻聚糖诱导类似于应激诱导的细胞凋亡样细胞死亡，而 褐藻聚糖则具有类似于应激诱导的坏死样细胞死亡的特征。不同岩藻聚糖在细胞内的通透性也存在差异。岩藻聚糖抗癌活性的这些差异适用于骨肉瘤的治疗。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c33a/7074035/0ebc0de74c15/marinedrugs-18-00104-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c33a/7074035/a770cfb20667/marinedrugs-18-00104-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c33a/7074035/597a41ad0672/marinedrugs-18-00104-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c33a/7074035/e3fffec8fc91/marinedrugs-18-00104-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c33a/7074035/c43c3574baa5/marinedrugs-18-00104-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c33a/7074035/6d807139223d/marinedrugs-18-00104-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c33a/7074035/2140f157dc95/marinedrugs-18-00104-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c33a/7074035/6dcd9203c202/marinedrugs-18-00104-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c33a/7074035/ab5c50634590/marinedrugs-18-00104-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c33a/7074035/0ebc0de74c15/marinedrugs-18-00104-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c33a/7074035/a770cfb20667/marinedrugs-18-00104-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c33a/7074035/597a41ad0672/marinedrugs-18-00104-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c33a/7074035/e3fffec8fc91/marinedrugs-18-00104-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c33a/7074035/c43c3574baa5/marinedrugs-18-00104-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c33a/7074035/6d807139223d/marinedrugs-18-00104-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c33a/7074035/2140f157dc95/marinedrugs-18-00104-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c33a/7074035/6dcd9203c202/marinedrugs-18-00104-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c33a/7074035/ab5c50634590/marinedrugs-18-00104-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c33a/7074035/0ebc0de74c15/marinedrugs-18-00104-g009.jpg

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