Department of Marine Bio-Food Sciences, Chonnam National University, Yeosu 59626, Korea.
Department of Marine Life Science, Jeju National University, Jeju 690-756, Korea.
Mar Drugs. 2020 Apr 8;18(4):196. doi: 10.3390/md18040196.
Fucoidans are biocompatible, heterogeneous, and fucose rich sulfated polysaccharides biosynthesized in brown algae, which are renowned for their broad-spectrum biofunctional properties. As a continuation of our preliminary screening studies, the present work was undertaken to extract polysaccharides from the edible brown algae by a modified enzyme assisted extraction process using Celluclast, a food-grade cellulase, and to purify fucoidan by DEAE-cellulose anion exchange chromatography. The apoptotic and antiproliferative properties of the purified fucoidan (F5) were evaluated on HL-60 and MCF-7 cells. Structural features were characterized by FTIR and NMR analysis. F5 indicated profound antiproliferative effects on HL-60 leukemia and MCF-7 breast cancer cells with IC values of 84.63 ± 0.08 µg mL and 93.62 ± 3.53 µg mL respectively. Further, F5 treatment increased the apoptotic body formation, DNA damage, and accumulation of HL-60 and MCF-7 cells in the Sub-G phase of the cell cycle. The effects were found to proceed via the mitochondria-mediated apoptosis pathway. The Celluclast assisted extraction is a cost-efficient method of yielding fucoidan. With further studies in place, purified fucoidan of could be applied as functional ingredients in food and pharmaceuticals.
褐藻中生物相容性、异质且富含岩藻糖的硫酸多糖，以其广泛的生物功能特性而闻名。作为我们初步筛选研究的延续，本工作采用纤维素酶 Celluclast 辅助酶解提取法从可食用褐藻中提取多糖，并通过 DEAE-纤维素阴离子交换层析对褐藻多糖进行纯化。对纯化的褐藻多糖（F5）在 HL-60 和 MCF-7 细胞上的凋亡和抗增殖特性进行了评价。通过傅里叶变换红外光谱（FTIR）和核磁共振（NMR）分析对其结构特征进行了表征。F5 对 HL-60 白血病和 MCF-7 乳腺癌细胞表现出显著的抗增殖作用，IC 值分别为 84.63 ± 0.08 µg/mL 和 93.62 ± 3.53 µg/mL。此外，F5 处理增加了 HL-60 和 MCF-7 细胞凋亡小体的形成、DNA 损伤以及细胞周期 S 期的积累。这些作用是通过线粒体介导的凋亡途径进行的。Celluclast 辅助提取是一种具有成本效益的褐藻多糖提取方法。随着进一步的研究，这种褐藻多糖有望作为功能性成分应用于食品和制药领域。
