Department of Information Engineering, I-Shou University, Kaohsiung 824, Taiwan.
Department of Emergency Medicine, E-Da Cancer Hospital, Kaohsiung 824, Taiwan.
Genes (Basel). 2019 Apr 20;10(4):309. doi: 10.3390/genes10040309.
Bacteria belonging to the genus are ubiquitously distributed in natural environments, plants, and animals. Except and , other species rarely cause human diseases. This study reported the whole-genome features, comparative genomic analysis, and antimicrobial susceptibility patterns of ED882-96 isolated in Taiwan. Strain ED882-96 was collected from the blood of a patient who had alcoholic liver cirrhosis and was an intravenous drug abuser. This isolate was initially identified as by using matrix-assisted laser desorption ionization-time of flight mass spectrometry. The analysis of 16S ribosomal RNA gene sequence revealed that ED882-96 shared 100% sequence identity with type strain CC-VM-7. The results of whole-genome sequencing of ED882-96 showed two chromosome contigs and one plasmid. The total lengths of the draft genomes of chromosome and plasmid were 4,249,864 bp and 435,667 bp, respectively. The findings of both in silico DNA-DNA hybridization and average nucleotide identity analyses clearly demonstrated that strain ED882-96 was a species of . A total of 83 potential virulence factor homologs were predicted in the whole-genome sequencing of strain ED882-96. This isolate was resistant to all tested antibiotics, including β-lactams, β-lactam/β-lactamase inhibitor combinations, aminoglycosides, fluoroquinolones, tetracycline, glycylcycline, and trimethoprim-sulfamethoxazole. Only one antibiotic resistance gene was recognized in the plasmid. By contrast, many antibiotic resistance genes were identified in the chromosome. The findings of this study suggest that strain ED882-96 is a highly virulent and multidrug-resistant pathogen. Knowledge regarding genomic characteristics and antimicrobial susceptibility patterns provides valuable insights into this uncommon species.
属于 属的细菌广泛分布于自然环境、植物和动物中。除 和 外，其他 种很少引起人类疾病。本研究报道了在台湾分离的 ED882-96 的全基因组特征、比较基因组分析和抗菌药物敏感性模式。菌株 ED882-96 从一名患有酒精性肝硬化和静脉药物滥用的患者的血液中分离得到。该分离株最初通过基质辅助激光解吸电离飞行时间质谱鉴定为 。16S 核糖体 RNA 基因序列分析表明，ED882-96 与 模式株 CC-VM-7 共享 100%的序列同一性。ED882-96 的全基因组测序结果显示有两个染色体片段和一个质粒。染色体和质粒的基因组草图长度分别为 4,249,864 bp 和 435,667 bp。DNA-DNA 杂交和平均核苷酸同一性分析的结果清楚地表明，菌株 ED882-96 是 属的一个种。在 ED882-96 的全基因组测序中预测了 83 个潜在的毒力因子同源物。该分离株对所有测试的抗生素均具有耐药性，包括β-内酰胺类、β-内酰胺/β-内酰胺酶抑制剂组合、氨基糖苷类、氟喹诺酮类、四环素类、甘氨环素类和甲氧苄啶-磺胺甲恶唑类。仅在质粒中识别到一个抗生素耐药基因。相比之下，在染色体中鉴定到许多抗生素耐药基因。本研究的结果表明，菌株 ED882-96 是一种高毒力和多药耐药的病原体。对基因组特征和抗菌药物敏感性模式的了解为这种罕见的物种提供了有价值的见解。
