Shibata Hirobumi, Nemoto Junpei, Shiba Shota, Yamane Yusuke, Kawai Gota, Hashimoto Kazuaki
Faculty of Engineering, Chiba Institute of Technology.
Photocatalysis International Research Center, Tokyo University of Science.
J Oleo Sci. 2020 Mar 1;69(3):271-276. doi: 10.5650/jos.ess19317. Epub 2020 Feb 13.
In this study, we investigated the direct detection of DNA, without pretreatment, using a quartz crystal microbalance (QCM) sensor. This sensor is modified by a self-assembled monolayer of a thiol derivative that has an amino group as the terminal functional group. Contact angle values and the attenuated total reflectance Fourier transform infrared (ATR/FT-IR) spectra of the QCM sensors after immersion into an ethanol solution of thiol derivatives clearly showed that self-assembled monolayers of the derivatives were formed on the QCM sensors. Although QCM measurements using unmodified and carboxylic group-modified sensors could not detect DNA-Na salt, the sensor modified with amino groups could detect the DNA. This system can be used for the analysis of the interaction between DNA and DNAbinding proteins.
在本研究中，我们研究了使用石英晶体微天平（QCM）传感器直接检测未经预处理的DNA。该传感器由具有氨基作为末端官能团的硫醇衍生物自组装单分子层修饰。将QCM传感器浸入硫醇衍生物的乙醇溶液后，其接触角值和衰减全反射傅里叶变换红外（ATR/FT-IR）光谱清楚地表明，衍生物的自组装单分子层在QCM传感器上形成。尽管使用未修饰和羧基修饰的传感器进行QCM测量无法检测到DNA钠盐，但氨基修饰的传感器可以检测到DNA。该系统可用于分析DNA与DNA结合蛋白之间的相互作用。
