Département de Microbiologie-Infectiologie et d'Immunologie, Université Laval, Québec City, Québec, Canada.
Pasteur Institute of Shanghai, China.
J Med Entomol. 2020 Jul 4;57(4):1025-1031. doi: 10.1093/jme/tjaa020.
Given current and projected changes in the climate, the composition of mosquito species is predicted to shift geographically with implications for the transmission dynamics of vector-borne pathogens. Many mosquito species are rarely collected in Canada and their history is poorly understood; thus assessing their potential role as vectors for pathogenesis is difficult. Mosquitoes were collected from four trapping sites in Quebec Province, Canada, from June to September during 2018 and 2019 using BG sentinel traps. From all morphologically identified female mosquitoes, at least one specimen was selected for identification confirmation using the DNA-barcoding technique. Sequences were subjected to alignment and a Neighbor-Joining (NJ) tree was created using Geneious software. In total, 2,752 female mosquitoes belonging to 20 species over five genera: including Aedes (Ae.), Anopheles (An.), Culex (Cx.), Culiseta (Cu.), Coquillettidia (Cq.) were collected. The predominant mosquito was found to be Ae. cinereus. The highest number of mosquito species was captured in July, followed by August, September, and then June. Five genera were characterized by a distinctive set of cytochrome oxidase I (COI) sequences that formed well-supported clusters in the NJ-tree. The presence of Ae.japonicus in Quebec provides an initial look at the distribution of mosquito species in eastern Canada, which may put Canadians at risk of a wider range of arboviruses.
鉴于当前和预计的气候变化，蚊子物种的组成预计将在地理上发生变化，这对病媒传播病原体的动态有影响。在加拿大，许多蚊子种类很少被采集，其历史也知之甚少；因此，评估它们作为病原体传播媒介的潜在作用是困难的。2018 年和 2019 年 6 月至 9 月期间，在加拿大魁北克省的四个诱捕点使用 BG 哨兵诱捕器收集了蚊子。从所有形态上鉴定为雌性的蚊子中，至少选择了一个标本，使用 DNA 条形码技术进行鉴定确认。对序列进行比对，并使用 Geneious 软件创建了邻接（NJ）树。总共收集了 2752 只属于五个属的 20 种雌性蚊子：包括埃及伊蚊（Ae.）、疟蚊属（An.）、库蚊属（Cx.）、库蚊属（Cu.）和库蚊属（Cq.）。优势蚊子是 Ae. cinereus。7 月捕获的蚊子种类最多，其次是 8 月、9 月，然后是 6 月。五个属的特征是一组独特的细胞色素氧化酶 I（COI）序列，在 NJ 树中形成了支持良好的聚类。日本伊蚊在魁北克的出现首次揭示了加拿大东部蚊子物种的分布情况，这可能使加拿大人面临更广泛的虫媒病毒风险。
