反相超高效液相色谱-电喷雾串联质谱法同时分析硫代葡萄糖苷和异硫氰酸酯。
Simultaneous Analysis of Glucosinolates and Isothiocyanates by Reversed-Phase Ultra-High-Performance Liquid Chromatography-Electron Spray Ionization-Tandem Mass Spectrometry.
机构信息
Laboratory of Food Chemistry, Wageningen University, P.O. Box 17, 6700 AA Wageningen, The Netherlands.
Department of Chemistry, Satya Wacana Christian University, Diponegoro 52-60, Salatiga 50711, Indonesia.
出版信息
J Agric Food Chem. 2020 Mar 11;68(10):3121-3131. doi: 10.1021/acs.jafc.9b07920. Epub 2020 Feb 27.
A new method to simultaneously analyze various glucosinolates (GSLs) and isothiocyanates (ITCs) by reversed-phase ultra-high-performance liquid chromatography-electron spray ionization-tandem mass spectrometry has been developed and validated for 14 GSLs and 15 ITCs. It involved derivatization of ITCs with -acetyl-l-cysteine (NAC). The limits of detection were 0.4-1.6 μM for GSLs and 0.9-2.6 μM for NAC-ITCs. The analysis of , , and extracts spiked with 14 GSLs and 15 ITCs indicated that the method generally had good intraday (≤10% RSD) and interday precisions (≤16% RSD). Recovery of the method was unaffected by the extracts and within 71-110% for GSLs and 66-122% for NAC-ITCs. The method was able to monitor the enzymatic hydrolysis of standard GSLs to ITCs in mixtures. Furthermore, GSLs and ITCs were simultaneously determined in Brassicaceae plant extracts before and after myrosinase treatment. This method can be applied to further investigate the enzymatic conversion of GSLs to ITCs in complex mixtures.
建立并验证了一种反相超高效液相色谱-电喷雾串联质谱法同时分析 14 种硫代葡萄糖苷（GSLs）和 15 种异硫氰酸酯（ITCs）的新方法，该方法涉及用 -乙酰-l-半胱氨酸（NAC）对 ITCs 进行衍生化。GSLs 的检测限为 0.4-1.6 μM，NAC-ITCs 的检测限为 0.9-2.6 μM。对添加了 14 种 GSLs 和 15 种 ITCs 的 、 、 提取物进行分析表明，该方法具有良好的日内（≤10%RSD）和日间精密度（≤16%RSD）。该方法的回收率不受提取物的影响，GSLs 的回收率为 71-110%，NAC-ITCs 的回收率为 66-122%。该方法能够监测标准 GSLs 在混合物中酶解生成 ITCs 的过程。此外，还在经过糜蛋白酶处理前后的芸薹属植物提取物中同时测定了 GSLs 和 ITCs。该方法可进一步用于研究复杂混合物中 GSLs 向 ITCs 的酶转化。
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