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肯尼亚感染胡子鲇(辐鳍鱼纲:胡子鲇科)的卡马鲶属线虫(线虫纲:卡马鲶科)的额外形态特征和分子数据

Additional Morphological Features and Molecular Data of (Nematoda: Camallanidae) Infecting (Actinopterygii: Clariidae) in Kenya.

作者信息

Rindoria Nehemiah Mogoi, Dos Santos Quinton Marco, Avenant-Oldewage Annemariè

机构信息

Department of Zoology, University of Johannesburg, P.O. Box 524, Auckland Park 2006, Johannesburg, South Africa.

出版信息

J Parasitol. 2020 Feb;106(1):157-166.

Abstract

This study provides additional taxonomic features based on scanning electron microscopy (SEM) and molecular data for ( Baylis, 1923 ) (Nematoda: Camallanidae). Parasites were collected from the posterior end of the intestine of cultured freshwater Sharptooth catfish (Burchell, 1822) from Kibos fish farm, Kisumu County, Kenya, from December 2017 to March 2018. Additional taxonomic features recorded for include the occurrence of 4-5 equal length digitate processes on the caudal end of the female, 4 processes (2 smaller and 2 larger) on the male caudal end, and a description of the shape of the distal tip of the right spicule. The study provides SEM images of the exposed sclerotized buccal capsule. This gives more information on the tridents, the sclerotized plate that extends laterally from the buccal capsule, and the narrow isthmus separating the anterior buccal capsule from the posterior. The prevalence, intensity, mean intensity, and mean abundance was 52.91%, 2-38, 12.37 and 6.54, respectively. rDNA fragments were amplified, sequenced, and compared to other camallanid taxa, and data confirmed the identity of the newly obtained sequences (MN396556) as that of being identical to sequence DQ813445 from Tanzania. This represents the first geographical record of in Kenya.

摘要

本研究基于扫描电子显微镜(SEM)和分子数据,为(Baylis,1923)(线虫纲:驼形线虫科)提供了更多分类学特征。2017年12月至2018年3月期间,从肯尼亚基苏木县基博斯养鱼场养殖的淡水尖齿胡鲇(Burchell,1822)的肠道后端采集了寄生虫。记录的关于该寄生虫的其他分类学特征包括:雌性尾端有4 - 5个等长的指状突起,雄性尾端有4个突起(2个较小和2个较大),以及对右交合刺远端尖端形状的描述。该研究提供了暴露的硬化口囊的扫描电子显微镜图像。这给出了关于三叉戟、从口囊横向延伸的硬化板以及将前口囊与后口囊分开的狭窄峡部的更多信息。其感染率、感染强度、平均感染强度和平均丰度分别为52.91%、2 - 38、12.37和6.54。对核糖体DNA片段进行了扩增、测序,并与其他驼形线虫类群进行了比较,分子数据证实新获得的序列(MN396556)与该寄生虫的身份一致,与来自坦桑尼亚的序列DQ813445相同。这代表了该寄生虫在肯尼亚的首次地理记录。

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