College of Animal Science and Technology, Anhui Agricultural University, Hefei, P. R. China.
New Rural Develop Research Institute, Anhui Agricultural University, Hefei, P. R. China.
PLoS One. 2020 Feb 13;15(2):e0228656. doi: 10.1371/journal.pone.0228656. eCollection 2020.
Fresh semen is most commonly used in an artificial insemination of small ruminants, because of low fertility rates of frozen sperm. Generally, when developing and applying assisted reproductive technologies, sheep and goats are classified as one species. In order to optimize sperm cryopreservation protocols in sheep and goat, differences in sperm proteomes between ram and buck are necessary to investigate, which may contribute to differences in function and fertility of spermatozoa. In the current work, a data-independent acquisition-mass spectrometry proteomic approach was used to characterize and make a comparison of ram (Ovis aries) and buck (Capra hircus) sperm proteomes. A total of 2,109 proteins were identified in ram and buck spermatozoa, with 238 differentially abundant proteins. Proteins identified in ram and buck spermatozoa are mainly involved in metabolic pathways for generation of energy and diminishing oxidative stress. Specifically, there are greater abundance of spermatozoa proteins related to the immune protective and capacity activities in ram, while protein that inhibit sperm capacitation shows greater abundance in buck. Our results not only provide novel insights into the characteristics and potential activities of spermatozoa proteins, but also expand the potential direction for sperm cryopreservation in ram and buck.
新鲜精液最常用于小反刍动物的人工授精，因为冷冻精子的受精率较低。通常，在开发和应用辅助生殖技术时，绵羊和山羊被归类为一个物种。为了优化绵羊和山羊精子的冷冻保存方案，有必要研究公羊和公山羊精子蛋白组之间的差异，这可能有助于解释精子功能和受精率的差异。在本研究中，采用数据非依赖性采集-质谱蛋白质组学方法对公羊（Ovis aries）和公山羊（Capra hircus）精子蛋白组进行了表征和比较。在公羊和公山羊精子中鉴定出了 2109 种蛋白质，其中有 238 种蛋白质丰度存在差异。鉴定出的公羊和公山羊精子蛋白主要参与产生能量和减少氧化应激的代谢途径。具体而言，在公羊精子中与免疫保护和活力相关的精子蛋白丰度更高，而在公山羊精子中抑制精子获能的蛋白丰度更高。我们的研究结果不仅为精子蛋白的特征和潜在活性提供了新的见解，而且为公羊和公山羊精子冷冻保存的潜在方向提供了扩展。
