Graduate School of Comprehensive Human Sciences, University of Tsukuba, Japan.
Japan Society for the Promotion of Science, Tokyo, Japan.
FEBS Open Bio. 2020 Jun;10(6):1005-1012. doi: 10.1002/2211-5463.12812. Epub 2020 Apr 21.
Renalase is predominantly expressed in the kidney, where it plays a role in catecholamine metabolism and blood pressure regulation. Moderate-intensity exercise (MEX) has been shown to increase the concentration of renalase in the blood and to reduce renal function in humans. Moreover, such exercise was also reported to increase catecholamine levels. Here, we examined renalase concentration in the blood and renalase expression levels in different organs after MEX in rats. Twelve male Wistar rats were made to run on a treadmill (MEX group) for 60 min at 20 m·min , after resting for 15 min. The control group rats were euthanized after resting on the treadmill. Tissue and blood samples were analyzed using western blotting, real-time RT-PCR and ELISA. Overall, the concentrations of renalase in the blood were significantly higher in the MEX group than that in the control group. Renalase expression was decreased in the kidney after 60 min of exercise, whereas the expression of renalase mRNA and protein in the extensor digitorum longus and plantaris muscles, respectively, increased after exercise. However, the expression of renalase in the other tissues examined did not change after acute exercise. In conclusion, we report that MEX for 60 min increases both renalase concentration in the blood and its expression in skeletal muscle.
肾酶主要在肾脏中表达，在儿茶酚胺代谢和血压调节中发挥作用。中等强度运动（MEX）已被证明可增加血液中肾酶的浓度，并降低人体的肾功能。此外，这种运动还被报道可增加儿茶酚胺水平。在这里，我们研究了大鼠 MEX 后血液中的肾酶浓度和不同器官中的肾酶表达水平。12 只雄性 Wistar 大鼠在 20 m·min 的跑步机上跑步 60 分钟（MEX 组），然后休息 15 分钟。对照组大鼠在跑步机上休息后被安乐死。使用 Western blot、实时 RT-PCR 和 ELISA 分析组织和血液样本。总的来说，MEX 组血液中的肾酶浓度明显高于对照组。运动 60 分钟后肾脏中的肾酶表达降低，而伸趾长肌和比目鱼肌中的肾酶 mRNA 和蛋白表达分别增加。然而，急性运动后其他检查组织中的肾酶表达没有变化。总之，我们报告称，60 分钟的中等强度运动可增加血液中的肾酶浓度及其在骨骼肌中的表达。
