乙醇可增强体外培养的大鼠纹状体和伏隔核切片中内源性多巴胺的钙依赖性刺激诱导释放。

Ethanol enhances the calcium-dependent stimulus-induced release of endogenous dopamine from slices of rat striatum and nucleus accumbens in vitro.

作者信息

Snape B M, Engel J A

机构信息

Department of Pharmacology, University of Göteborg, Sweden.

出版信息

Neuropharmacology. 1988 Nov;27(11):1097-101. doi: 10.1016/0028-3908(88)90003-2.

DOI:10.1016/0028-3908(88)90003-2

PMID:3205382

Abstract

When slices of striatum from the rat were preincubated for 40 min in calcium-free medium in vitro, the subsequent release of endogenous dopamine (DA) induced by 40 mM KCl was completely calcium-dependent, showing a maximal response in the presence of 1.5 mM CaCl2, and a half-maximal response in the presence of 0.5 mM CaCl2. In this calcium-dependent preparation, ethanol, at concentrations of 80-120 mM, significantly increased the KCl-induced release of endogenous DA from the striatum by 19-29%. This effect was not reproducible in calcium-replete incubation medium (2.0 mM CaCl2), suggesting that the phenomenon was calcium-dependent. Preliminary studies in the nucleus accumbens of the rat showed a similar increase (30%) in the calcium-dependent, KCl-induced release of endogenous DA in the presence of 100 mM ethanol.

摘要

当将大鼠纹状体切片在无钙培养基中体外预孵育40分钟时，随后由40 mM氯化钾诱导的内源性多巴胺（DA）释放完全依赖于钙，在存在1.5 mM氯化钙时显示出最大反应，在存在0.5 mM氯化钙时显示出半最大反应。在这种依赖钙的制剂中，浓度为80 - 120 mM的乙醇显著增加了纹状体中氯化钾诱导的内源性DA释放，增幅为19 - 29%。在富含钙的孵育培养基（2.0 mM氯化钙）中这种效应无法重现，表明该现象依赖于钙。对大鼠伏隔核的初步研究表明，在存在100 mM乙醇的情况下，依赖钙的、由氯化钾诱导的内源性DA释放也有类似的增加（30%）。