Zaragoza-Bastida Adrian, Flores-Aguilar Saudy Consepcion, Aguilar-Castro Liliana Mireya, Morales-Ubaldo Ana Lizet, Valladares-Carranza Benjamín, Rangel-López Lenin, Olmedo-Juárez Agustín, Rosenfeld-Miranda Carla E, Rivero-Pérez Nallely
Universidad Autónoma del Estado de Hidalgo, Área Académica de Medicina Veterinaria y Zootecnia, Instituto de Ciencias Agropecuarias, Rancho Universitario Av. Universidad km 1, EX-Hda de Aquetzalpa, Tulancingo, Hidalgo 43600, México.
Universidad Autónoma del Estado de Hidalgo, Área Académica de Biología, Instituto de Ciencias Básicas e Ingeniería. Carretera Pachuca-Tulancingo S/N Int. 22 Colonia Carboneras, Mineral de la Reforma, Hidalgo 42180, México.
Animals (Basel). 2020 Feb 11;10(2):281. doi: 10.3390/ani10020281.
Rattlesnakes have venoms with a complex toxin mixture comprised of polypeptides and proteins. Previous studies have shown that some of these polypeptides are of high value for the development of new medical treatments. The aim of the present study is to evaluate, in vitro, the antibacterial and hemolytic activity of and venoms. A direct field search was conducted to obtain and venom samples. These were evaluated to determine their antibacterial activity against , and through the techniques of Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC). Hemolytic activity was also determined. Antibacterial activity was determined for treatments ( 2) CT2 and ( 3) CR3, obtaining a Minimum Inhibitory Concentration of 50 µg/mL and a Minimum Bactericidal Concentration of 100 µg/mL against . CT1 ( 1), CT2, and CR3 presented hemolytic activity; on the other hand, 4 (CR4) did not show hemolytic activity. The results of the present study indicate for the first time that Crotalus triseriatus and Crotalus ravus venoms contain some bioactive compounds with bactericidal activity against Pseudomonas aeruginosa which could be used as alternative treatment in diseases caused by this pathogenic bacterium.
响尾蛇的毒液含有由多肽和蛋白质组成的复杂毒素混合物。先前的研究表明，其中一些多肽对开发新的医学治疗方法具有很高的价值。本研究的目的是在体外评估菱斑响尾蛇和墨西哥角响尾蛇毒液的抗菌和溶血活性。通过直接在野外搜寻来获取菱斑响尾蛇和墨西哥角响尾蛇的毒液样本。通过最低抑菌浓度（MIC）和最低杀菌浓度（MBC）技术对这些样本进行评估，以确定它们对铜绿假单胞菌、金黄色葡萄球菌和大肠杆菌的抗菌活性。同时也测定了溶血活性。对处理组（2）CT2和（3）CR3的抗菌活性进行了测定，其对铜绿假单胞菌的最低抑菌浓度为50μg/mL，最低杀菌浓度为100μg/mL。CT1（1）、CT2和CR3呈现出溶血活性；另一方面，4（CR4）没有表现出溶血活性。本研究结果首次表明，菱斑响尾蛇和墨西哥角响尾蛇的毒液含有一些对铜绿假单胞菌具有杀菌活性的生物活性化合物，可作为这种病原菌引起的疾病的替代治疗方法。
