García Hernández Leslie Cynthia, Higuera-Piedrahita Rosa Isabel, Rivero-Perez Nallely, Morales-Ubaldo Ana Lizet, Valladares-Carranza Benjamín, de la Cruz-Cruz Héctor Alejandro, Cuéllar-Ordaz Jorge Alfredo, González-Ruiz Cynthia, Nicolás-Vázquez María Inés, Zaragoza-Bastida Adrian
Laboratorio 3 de la Unidad de Investigación Multidisciplinaria. Facultad de Estudios Superiores Cuautitlán, Universidad Nacional Autónoma de México, Carretera Cuautitlán-Teoloyucan km 2.5, San Sebastián Xhala, Cuautitlán 54714, Estado de México, Mexico.
Área Académica de Medicina Veterinaria y Zootecnia, Instituto de Ciencias Agropecuarias, Universidad Autónoma del Estado de Hidalgo, Rancho Universitario Av. Universidad km. 1, Ex Hacienda de Aquetzalpa, Tulancingo de Bravo 43660, Hidalgo, Mexico.
Pharmaceuticals (Basel). 2025 May 23;18(6):781. doi: 10.3390/ph18060781.
The World Health Organization notes that some bacteria have been demonstrated to possess significant public health risks; they have antibiotic resistance, and there are fewer alternatives for control. The -hexane extract and cinaguaiacin obtained from show promising antibacterial activity, including against multidrug-resistant bacteria that affect animal and human health. The aim of this study was to determine the antibacterial activity of the -hexane extract of and cinaguaiacin against multidrug-resistant bacteria. was collected in the pre-flowering period, the -hexane extract was obtained, and chromatographic techniques and structure were used to separate the lignans, which were elucidated with nuclear magnetic resonance techniques. Four ATCC strains were used, and four strains were isolated from clinical cases with different resistance profiles. The antibacterial activity was determined by calculating the Minimum Inhibitory Concentration (MIC), Minimum Bactericidal Concentration (MBC), the time-kill kinetics assay, and the cell membrane integrity and DNA release assay. Molecular docking studies of lignans demonstrated the binding mode involved in the active site of DNA gyrase B. The -hexane extract inhibited growth against 87.5% of the strains tested (MIC 5.31 to 42.5 mg/mL) and showed bactericidal activity against 25% of the strains tested (MBC 0.62 to 85 mg/mL). Cinaguaiacin inhibited growth against 100% of the strains tested (MIC, 0.56 to 2.25 mg/mL) and exhibited bactericidal activity against 25% of the strains tested (MBC, 0.62 to 85 mg/mL). The mechanism of cinaguaiacin's action may be associated with damage to the plasma membrane, as the protein and DNA levels were higher than those of the positive control. The n-hexane extract and cinaguaiacin obtained from showed a bacteriostatic or bactericidal effect, depending on the strain evaluated.
世界卫生组织指出，一些细菌已被证明具有重大的公共卫生风险；它们具有抗生素抗性，且控制手段较少。从[植物名称]中获得的正己烷提取物和肉桂愈创木酚表现出有前景的抗菌活性，包括对影响动物和人类健康的多重耐药菌。本研究的目的是确定[植物名称]的正己烷提取物和肉桂愈创木酚对多重耐药菌的抗菌活性。[植物名称]在开花前期采集，获得正己烷提取物，采用色谱技术和结构分析来分离木脂素，并用核磁共振技术对其进行阐明。使用了四株美国典型培养物保藏中心（ATCC）菌株，以及从具有不同耐药谱的临床病例中分离出的四株菌株。通过计算最低抑菌浓度（MIC）、最低杀菌浓度（MBC）、时间杀菌动力学试验以及细胞膜完整性和DNA释放试验来确定抗菌活性。木脂素的分子对接研究证明了其与DNA旋转酶B活性位点的结合模式。正己烷提取物对87.5%的测试菌株有生长抑制作用（MIC为5.31至42.5毫克/毫升），对25%的测试菌株有杀菌活性（MBC为0.62至85毫克/毫升）。肉桂愈创木酚对100%的测试菌株有生长抑制作用（MIC为0.56至2.25毫克/毫升），对25%的测试菌株有杀菌活性（MBC为0.62至85毫克/毫升）。肉桂愈创木酚的作用机制可能与对质膜的损伤有关，因为蛋白质和DNA水平高于阳性对照。从[植物名称]中获得的正己烷提取物和肉桂愈创木酚根据所评估的菌株表现出抑菌或杀菌作用。
