Área Académica de Medicina Veterinaria y Zootecnia, Instituto de Ciencias Agropecuarias, Universidad Autónoma del Estado de Hidalgo, Rancho Universitario Av. Universidad Km 1, EX-Hda de Aquetzalpa, Tulancingo 43600, Hidalgo, Mexico.
Centro de Investigación Biomédica del Sur, Instituto Mexicano del Seguro Social, Argentina No. 1. Col. Centro, Xochitepec 62790, Morelos, Mexico.
Molecules. 2022 Jun 5;27(11):3620. doi: 10.3390/molecules27113620.
Bovine mastitis is one of the most common diseases in dairy cows, and it causes significant economic losses in dairy industries worldwide. Gram-positive and Gram-negative bacteria can cause bovine mastitis, and many of them have developed antimicrobial resistance. There is an urgent need for novel therapeutic options to treat the disease. -derived compounds represent an important potential alternative treatment. The aim of the present study was to isolate and characterize antibacterial compounds from against multidrug-resistant bacteria associated with bovine mastitis. The hydroalcoholic extract (LTHE) exhibited antibacterial activity. The extract was subjected to a bipartition, giving an aqueous fraction (moderate antibacterial activity) and an organic fraction (higher antibacterial activity). Chromatographic separation of the organic fraction enabled us to obtain four active sub-fractions. Chemical analyses through HPLC techniques were conducted for the LTHE, fractions, and sub-fraction Ltc1-F3, from which we isolated two compounds, characterized by H and C NMR analyses. Compound -3 demethoxyisoguaiacin exhibited the best antibacterial activity against the evaluated bacteria (MIC: 0.01-3.12 mg/mL; MBC: 0.02-3.12 mg/mL). The results indicated that -3 demethoxyisoguaiacin can be used as an alternative treatment for multidrug-resistant bacteria associated with mastitis.
奶牛乳腺炎是奶牛中最常见的疾病之一，它会给全球的奶制品行业造成巨大的经济损失。革兰氏阳性菌和革兰氏阴性菌都能引起奶牛乳腺炎，其中许多细菌已经产生了抗药性。因此，我们迫切需要新的治疗方法来治疗这种疾病。来源于植物的化合物代表了一种重要的潜在替代治疗方法。本研究的目的是从 中分离和鉴定具有抗多药耐药菌活性的抗菌化合物，用于治疗与奶牛乳腺炎相关的多药耐药菌。 水醇提取物（LTHE）表现出抗菌活性。将提取物进行二部分分离，得到水相部分（具有中等抗菌活性）和有机相部分（具有更高抗菌活性）。对有机相部分进行色谱分离，得到四个具有活性的亚部分。通过 HPLC 技术对 LTHE、各馏分和亚馏分 Ltc1-F3 进行化学分析，从其中分离出两种化合物，通过 1H 和 13C NMR 分析对其进行了表征。化合物 -3 去甲氧基异喹啉具有最好的抗菌活性，对评估的细菌的 MIC 值为 0.01-3.12mg/mL；MBC 值为 0.02-3.12mg/mL。结果表明，-3 去甲氧基异喹啉可作为治疗乳腺炎相关多药耐药菌的替代药物。
