超量饲喂的溆浦鹅和朗德鹅在生产性能、脂肪酸组成、与脂质代谢相关的酶及基因表达方面的比较
Comparison of overfed Xupu and Landes geese in performance, fatty acid composition, enzymes and gene expression related to lipid metabolism.
作者信息
Liu Xu, Li Peng, He Changqing, Qu Xiangyong, Guo Songchang
机构信息
College of Animal Science and Technology, Hunan Agricultural University, Changsha, Hunan 410128, China.
Hunan Engineering Research Center of Poultry Production Safety, Hunan 410128, China.
出版信息
Asian-Australas J Anim Sci. 2020 Dec;33(12):1957-1964. doi: 10.5713/ajas.19.0842. Epub 2020 Jan 13.
OBJECTIVE
The aim of this study was to compare overfeeding performance, fatty acid composition, blood chemistry, enzymes and genes expression overfed Xupu and Landes geese.
METHODS
Sixty male Xupu geese (80 d) and Landes geese (80 d) were selected. After a period of one-week of pre-overfeeding, Xupu and Landes geese were overfed three meals of 550 and 350 g/d, respectively, of a high-carbohydrate diet in the first week of the overfeeding period. The next week, geese were given four meals of 1,200 and 850 g/d, respectively, over 8 to 14 d. Finally, geese were given five meals of 1,600 and 1,350 g/d, respectively, for the last two weeks.
RESULTS
After overfeeding for 28 d: Compared with Landes geese, Xupu geese liver weight and liver-to-body weight ratio decreased (p<0.05), while final weight, slaughter weight, total weight gain, abdominal fat weight, and feed-to-liver weight ratio increased (p<0.05). The levels of elaidic acid (C18:1t9), oleic acid (C18:1n-9), eicosenoic acid, and arachidonic acid in the liver of Xupu geese significantly increased (p<0.05), and the levels of myristic acid and stearic acid significantly decreased (p<0.05), while methyleicosanoate acid significantly increased (p<0.05). Xupu geese had higher plasma concentrations of triglyceride and very low density lipoprotein cholesterol (p<0.05), and decreased activities of alanine aminotransferase, aspartate aminotransferase, and lipase (LPS) (p<0.05). Landes geese had higher LPS activity (p<0.05), but lower cholinesterase activity (p<0.05) when compared with Xupu geese. The mRNA expression levels of fatty acid dehydrogenase (FADS) gene, elongase of longchain fatty acid 1 (ELOVL1) gene, ELOVL5, and acyl-Co A: cholesterol acyltransferase 2 (ACAT2) gene were significantly upregulated (p<0.05) in Landes goose when compared with Xupu geese.
CONCLUSION
This study demonstrates that the liver production performance of Landes geese was better than that of Xupu geese to some extent, which may be closely related to LPS activity, as well as the expression of FADS, ELOVL1, ELOVL5, and ACAT2.
目的
本研究旨在比较溆浦鹅和朗德鹅过度饲喂后的生长性能、脂肪酸组成、血液生化指标、酶活性及基因表达情况。
方法
选取60只80日龄的雄性溆浦鹅和朗德鹅。在过度饲喂前进行为期一周的预饲期，之后在过度饲喂期的第一周，溆浦鹅和朗德鹅分别每日分三餐过量饲喂550克和350克高碳水化合物日粮。第二周，鹅分别每日分四餐，在8至14天内过量饲喂1200克和850克。最后两周，鹅分别每日分五餐，过量饲喂1600克和1350克。
结果
过度饲喂28天后：与朗德鹅相比，溆浦鹅的肝脏重量和肝体比降低（p<0.05），而末重、屠宰重、总增重、腹脂重和料肝比增加（p<0.05）。溆浦鹅肝脏中反油酸（C18:1t9）、油酸（C18:1n-9）、二十碳烯酸和花生四烯酸水平显著升高（p<0.05），肉豆蔻酸和硬脂酸水平显著降低（p<0.05），而甲基二十烷酸显著增加（p<0.05）。溆浦鹅血浆中甘油三酯和极低密度脂蛋白胆固醇浓度较高（p<0.05），丙氨酸氨基转移酶、天冬氨酸氨基转移酶和脂肪酶（LPS）活性降低（p<0.05）。与溆浦鹅相比，朗德鹅LPS活性较高（p<0.05），但胆碱酯酶活性较低（p<0.05）。与溆浦鹅相比，朗德鹅脂肪酸脱氢酶（FADS）基因、长链脂肪酸延长酶1（ELOVL1）基因、ELOVL5和酰基辅酶A：胆固醇酰基转移酶2（ACAT2）基因的mRNA表达水平显著上调（p<0.05）。
结论
本研究表明，朗德鹅的肝脏生产性能在一定程度上优于溆浦鹅，这可能与LPS活性以及FADS、ELOVL1、ELOVL5和ACAT2的表达密切相关。
Zotero 插件