多组学分析为水禽脂肪肝形成的分子机制提供了新的见解。

Multi-omics analysis provides new insights into molecular mechanisms for waterfowl fatty liver formation.

作者信息

Qi Jingjing, Li Junpeng, Xi Yang, Yang Zhao, Guo Shihao, Han Xu, Wang Rui, Li Liang, Bai Lili, Han Chunchun, Wang Jiwen, Liu Hehe

机构信息

State Key Laboratory of Swine and Poultry Breeding Industry, College of Animal Science and Technology, Sichuan Agricultural University, PR China; Key Laboratory of Livestock and Poultry Multi-omics, Ministry of Agriculture and Rural Affairs, College of Animal Science and Technology, Sichuan Agricultural University, PR China; Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan Agricultural University, PR China.

出版信息

Poult Sci. 2025 Jun 6;104(9):105398. doi: 10.1016/j.psj.2025.105398.

DOI:10.1016/j.psj.2025.105398

PMID:40570459

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC12241975/

Abstract

Waterfowl fatty liver has high nutritional value and offers benefits to human health. While previous research on fatty liver has focused on individual organs, this study explored fatty liver by examining the cecum, serum metabolites, and liver gene expression. This study integrated transcriptomic, metabolomic, and 16S rRNA microbiome analyses to analyze the molecular mechanism of waterfowl fatty liver formation. We identified seven core genes, five core metabolites, and three core microorganisms, which were significantly correlated. Overfeeding increased the abundance of Mucispirillum in the cecum, while Prevotella and Olsenella decreased. These microbial shifts, mediated by metabolites such as phthalic acid, influenced lipid metabolism, which induced changes in liver gene expression, including upregulation of ACBD4 and downregulation of HSP90B1 and HSPA5, thereby supporting fatty liver development. Additionally, ABC transporters, protein processing in the endoplasmic reticulum, and amino acid metabolism were important in fatty liver development. Our research findings provide new insights into the molecular mechanism of fatty liver with overfeeding in waterfowl from the perspective of the gut-liver axis.

摘要

水禽脂肪肝具有很高的营养价值，对人体健康有益。虽然以往关于脂肪肝的研究主要集中在单个器官上，但本研究通过检测盲肠、血清代谢物和肝脏基因表达来探究脂肪肝。本研究整合了转录组学、代谢组学和16S rRNA微生物组分析，以分析水禽脂肪肝形成的分子机制。我们鉴定出七个核心基因、五种核心代谢物和三种核心微生物，它们之间存在显著相关性。过度喂食增加了盲肠中黏液螺旋菌的丰度，而普雷沃氏菌和奥尔森氏菌减少。这些由邻苯二甲酸等代谢物介导的微生物变化影响了脂质代谢，进而诱导肝脏基因表达发生变化，包括ACBD4上调以及HSP90B1和HSPA5下调，从而促进了脂肪肝的发展。此外，ABC转运蛋白、内质网中的蛋白质加工以及氨基酸代谢在脂肪肝发展过程中也很重要。我们的研究结果从肠-肝轴的角度为水禽过度喂食导致脂肪肝的分子机制提供了新的见解。