Ran Ke, Li Qi, Qi Lu, Li Weidong, Kong Lingfeng
Key Laboratory of Mariculture, Ministry of Education, Ocean University of China, Qingdao, China.
Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, Qingdao, China.
Mitochondrial DNA A DNA Mapp Seq Anal. 2020 Mar;31(2):57-63. doi: 10.1080/24701394.2020.1726898. Epub 2020 Feb 13.
A number of same species of Cerithiidae are morphologically unlike, whereas most of species in the same genus are morphologically similar and just exhibit subtle differences. It is difficult to identify them by morphological methods alone. DNA barcoding is a modern molecular technique that can be used to identify species accurately, and is particularly helpful when distinguishing morphologically similar species. In order to identify species of Cerithiidae using DNA barcoding technology based on mitochondrial cytochrome oxidase subunit I (COI) and 16S ribosomal RNA (16S rRNA) genes, this study calculated intraspecific and interspecific genetic distance and constructed the phylogenetic trees. A total of 80 COI and 16S rRNA barcode sequences were obtained from 10 species and 3 genera. Some unknown specimens were further identified and a cryptic species may exist in , showing that DNA barcoding technology has the potential to discover new species and cryptic species. The phylogenetic trees revealed that all of the cerithiids could converge upon a monophyly with high support values and two genera ( and ) maybe support the reclassification. It is necessary for traditional morphological methods to combine with the DNA barcoding for classification and identification of Cerithiidae.
许多同属蜒螺科的物种在形态上存在差异，而同一属中的大多数物种在形态上相似，仅表现出细微差别。仅通过形态学方法很难对它们进行鉴别。DNA条形码技术是一种现代分子技术，可用于准确识别物种，在区分形态相似的物种时尤其有用。为了利用基于线粒体细胞色素氧化酶亚基I（COI）和16S核糖体RNA（16S rRNA）基因的DNA条形码技术鉴别蜒螺科物种，本研究计算了种内和种间遗传距离并构建了系统发育树。从3个属的10个物种中总共获得了80条COI和16S rRNA条形码序列。一些未知标本得到了进一步鉴别，并且可能存在一个隐存种，这表明DNA条形码技术有发现新物种和隐存种的潜力。系统发育树显示，所有蜒螺科物种都能在高支持值下汇聚成一个单系群，并且两个属（和）可能支持重新分类。传统形态学方法与DNA条形码技术相结合对于蜒螺科的分类和鉴别是必要的。
