Samadi Mahsan, Tabibian Farinaz, Moradzadeh Kobra, Nassiri Seyed Mahdi, Gheisari Yousof
Isfahan Student Research Center, Isfahan University of Medical Sciences, Isfahan, Iran.
Department of Genetics and Molecular Biology, Isfahan University of Medical Sciences, Isfahan, Iran.
J Res Med Sci. 2020 Jan 20;25:6. doi: 10.4103/jrms.JRMS_249_19. eCollection 2020.
Acute kidney injury is a high-risk complication in a variety of clinical situations mostly due to ischemia-reperfusion (IR) injuries. The novel idea of remote ischemic preconditioning (rIPC) was proposed to prevent serious ischemia sequels. To address the controversy of previous reports, the current study was performed to assess the effect of rIPC on kidney IR injury.
Male BALB/c mice were exposed to either rIPC or sham intervention, 24 h before kidney IR. In two independent sets of experiments, rIPC was accomplished by inducing three cycles of 5 min ischemia with 5 min reperfusion intervals through the ligation of the left external iliac artery or infrarenal abdominal aorta. Kidney IR injury was performed by left renal pedicle occlusion for 35 min and simultaneous right nephrectomy. After 48 h, mice were sacrificed for the assessment of kidney function and structure.
According to the serum urea and creatinine, as well as histopathological measures, none of the exploited rIPC procedures could significantly protect against kidney IR injury.
Based on our findings and the divergent results of previous animal and human studies, it can be concluded that the renoprotective effects of rIPC are minimal, if any, and are not robustly detectable.
急性肾损伤是多种临床情况下的高风险并发症，主要归因于缺血再灌注（IR）损伤。远程缺血预处理（rIPC）这一新颖概念被提出用于预防严重的缺血后遗症。为解决先前报道中的争议，开展了本研究以评估rIPC对肾脏IR损伤的影响。
雄性BALB/c小鼠在肾脏IR前24小时接受rIPC或假干预。在两组独立实验中，通过结扎左髂外动脉或肾下腹主动脉诱导三个5分钟缺血周期，间隔5分钟再灌注来完成rIPC。通过结扎左肾蒂35分钟并同时切除右肾来造成肾脏IR损伤。48小时后，处死小鼠以评估肾功能和结构。
根据血清尿素和肌酐以及组织病理学指标，所采用的rIPC程序均不能显著保护肾脏免受IR损伤。
基于我们的研究结果以及先前动物和人体研究的不同结果，可以得出结论，rIPC的肾脏保护作用即便存在也是微乎其微的，且无法可靠地检测到。
