Zhang Xuebin, Liu Jiayu, Yan Xiaoling
Department of Pathology, Tianjin Huanhu Hospital Tianjin, P. R. China.
Department of Neurosurgery, Peking University People's Hospital 11th Xizhimen South Street, Beijing, P. R. China.
Int J Clin Exp Pathol. 2020 Jan 1;13(1):44-48. eCollection 2020.
Intraoperative pathologic diagnosis for central nervous system (CNS) tumors is important to determine the neurosurgery procedure. But sometimes the differential diagnosis between glioma and lymphoma, or glioma and metastatic tumors is difficult for a pathologist during a short time, especially when the specimen is small or the frozen section has ice crystals. Immunohistochemistry (IHC) is a very useful method for diagnosis, but the traditional immunohistochemical method is time-consuming and not suitable intraoperatively. In this study, we chose Cytokeratin-pan, GFAP, and LCA as three immunohistochemical indicators. Intraoperative IHC was done by Novodiax ihcDirect technology combined with Leica Bond auto-staining. Compared with the manual method recommended for the reagents (Novodiax ihcDirect), the results show that auto-staining has better stability and high reproducibility in coloration, which has broad prospects for future application.
中枢神经系统（CNS）肿瘤的术中病理诊断对于确定神经外科手术程序很重要。但有时病理学家在短时间内难以对胶质瘤与淋巴瘤，或胶质瘤与转移瘤进行鉴别诊断，尤其是当标本较小或冰冻切片有冰晶时。免疫组织化学（IHC）是一种非常有用的诊断方法，但传统的免疫组织化学方法耗时且不适合术中使用。在本研究中，我们选择细胞角蛋白全谱、胶质纤维酸性蛋白（GFAP）和白细胞共同抗原（LCA）作为三种免疫组织化学指标。术中免疫组织化学采用Novodiax ihcDirect技术结合徕卡Bond自动染色完成。与试剂推荐的手动方法（Novodiax ihcDirect）相比，结果表明自动染色在染色方面具有更好的稳定性和高重复性，具有广阔的未来应用前景。
