Dong Xiaohong, Rao Dawei, Tian Lejin, Wang Qizheng, Yang Kun
Department of Pharmaceutical & Biological Engineering, School of Chemical Engineering, Sichuan University, Chengdu, 610065, China.
Heliyon. 2020 Feb 4;6(2):e03348. doi: 10.1016/j.heliyon.2020.e03348. eCollection 2020 Feb.
Antibiotics released in the environment have attracted great attention. The environmental emission control of antibiotics should be based on the degree of their negative impacts on the environment and ecology. Here, we conducted a series of soil slurry microcosm experiments to investigate the interactions between antibiotics and the soil bacterial community. In the soil slurry, distinctive behaviors were observed for different antibiotics. Beta-lactams (ampicillin and ceftriaxone) experienced fast biodegradation. Kanamycin was adsorbed on soil particles soon after its addition. Nalidixic acid was stable throughout the experimental period (164 h). The main inactivation mechanism of tetracycline was deduced to be hydrolysis. Bacterial communities in slurries with or without antibiotic-treatment were profiled high-throughput Illumina sequencing of the 16S rRNA gene. Unstable (ceftriaxone) and adsorbed (kanamycin) antibiotics show minor or negligible influences on the soil bacterial community. Stable antibiotics (nalidixic acid and tetracycline) have significantly affected the structure of the bacterial community. Most of enriched bacterial genera by various antibiotics belong to the same phylum, . Inhibited bacterial phyla by nalidixic acid are and , while those inhibited by tetracycline are , and . According to the PICRUSt prediction of metagenome, influence of antibiotics on overall metabolic function of the bacterial community is rather limited. This study has provided valuable information, from a phylogenetic viewpoint, about the influence of high concentration of antibiotics on soil bacterial community.
环境中释放的抗生素已引起广泛关注。抗生素的环境排放控制应基于其对环境和生态的负面影响程度。在此，我们进行了一系列土壤泥浆微观实验，以研究抗生素与土壤细菌群落之间的相互作用。在土壤泥浆中，不同抗生素表现出不同的行为。β-内酰胺类抗生素（氨苄青霉素和头孢曲松）快速生物降解。卡那霉素添加后很快被土壤颗粒吸附。萘啶酸在整个实验期间（164小时）保持稳定。四环素的主要失活机制推断为水解。通过对16S rRNA基因进行高通量Illumina测序，分析了添加或未添加抗生素处理的泥浆中的细菌群落。不稳定的（头孢曲松）和被吸附的（卡那霉素）抗生素对土壤细菌群落的影响较小或可忽略不计。稳定的抗生素（萘啶酸和四环素）对细菌群落结构有显著影响。各种抗生素富集的大多数细菌属属于同一门。萘啶酸抑制的细菌门是 和 ，而四环素抑制的是 、 和 。根据宏基因组的PICRUSt预测，抗生素对细菌群落整体代谢功能的影响相当有限。本研究从系统发育的角度提供了关于高浓度抗生素对土壤细菌群落影响的有价值信息。
