Coates Jessica L, Lawson Ashanti J, Bostick Kathleen, Ayalew Mentewab
Biology Department, Spelman College, 350 Spelman Lane, Atlanta, GA, 30314, USA.
Environ Evid. 2025 Jul 10;14(1):12. doi: 10.1186/s13750-025-00365-6.
Exposure to aminoglycosides, a class of potent bactericidal antibiotics naturally produced by soil microorganisms and commonly used in agriculture, has the potential to cause shifts in the population dynamics of microorganisms that impact plant and soil health. In particular, aminoglycoside exposure could result in alterations of the soil and plant root-associated bacterial species diversity and richness due to their potent inhibitory action on microbial growth, the creation of selective conditions for the proliferation of antibiotic-resistant bacteria, or a reduction in the ability to suppress soil pathogens. Previous studies have attempted to understand the relationship between aminoglycoside exposure and the plant-associated microbiota with varying results. Thus, this systematic review aims to survey all relevant published data to answer the question, "What is the impact of aminoglycoside exposure on the soil and plant root-associated microbiota?"
We searched 5 academic databases and 1 specialist organization database for scientific journal publications written in any language. Articles were included based on the criteria described in Coates et al., 2022. Included studies were subject to critical appraisal using the CEE Critical Appraisal Tool Version 0.2 (Prototype) to evaluate their susceptibility to confounding factors, misclassification bias, selection bias, attrition bias, reporting bias and analysis bias. Studies deemed to be high risk based on critical appraisal results were excluded from further analysis. Descriptive data analysis was performed for studies considered low or unclear for risk of bias. Meta-analyses were conducted for antibiotic resistance and microbial diversity.
Out of 8370 screened records, 50 articles fulfilled the search criteria, and from these, 13 studies were included in meta-analysis. Most studies investigated the impact of aminoglycoside exposure on soil microbiota (93%) in a laboratory setting (62%), primarily from the United States (32%), China (24%), France, Switzerland and Germany (8%). A limited number of studies investigated the impact of aminoglycoside exposure on disease suppression, so it was excluded from meta-analysis. Therefore, our synthesis primarily details the impact of aminoglycoside exposure on the microbial diversity and antibiotic resistance of the soil microbiota. Overall, exposure to aminoglycosides did not result in a significant change in the microbial diversity. However, soil use, pH, and type of aminoglycoside used could be potential modifiers. Additionally, we observed an average 7% of the microbial population exhibiting resistance to aminoglycosides, with the relationship between the exposure concentration and the selection concentration emerging as a potential modifier.
Current research is limited by gaps in understanding the relationship between aminoglycoside exposure, microbial community dynamics, and disease suppression, as well as by insufficient data on less-studied aminoglycosides and key confounding factors. Current research also suggests a potential relationship between antibiotic concentrations used for exposure and selection of resistant bacteria. These findings emphasize the need for informed antibiotic management policies and rigorous, targeted research to better understand the relationship between soil factors and antibiotic concentrations used on the impact of aminoglycosides on soil microbiota.
氨基糖苷类抗生素是一类由土壤微生物天然产生的强效杀菌抗生素，常用于农业生产，接触此类抗生素可能导致微生物种群动态变化，进而影响植物和土壤健康。特别是，氨基糖苷类抗生素的接触可能会改变土壤和植物根系相关细菌的物种多样性和丰富度，这是因为它们对微生物生长具有强大的抑制作用，为抗生素抗性细菌的增殖创造了选择性条件，或者降低了抑制土壤病原体的能力。以往的研究试图了解氨基糖苷类抗生素接触与植物相关微生物群之间的关系，但结果各异。因此，本系统综述旨在调查所有相关的已发表数据，以回答“氨基糖苷类抗生素接触对土壤和植物根系相关微生物群有何影响？”这一问题。
我们在5个学术数据库和1个专业组织数据库中搜索了任何语言撰写的科学期刊出版物。根据Coates等人2022年描述的标准纳入文章。使用CEE批判性评价工具版本0.2（原型）对纳入的研究进行批判性评价，以评估其对混杂因素、错误分类偏差、选择偏差、损耗偏差、报告偏差和分析偏差的敏感性。根据批判性评价结果被判定为高风险的研究被排除在进一步分析之外。对被认为偏倚风险低或不明确的研究进行描述性数据分析。对抗生素抗性和微生物多样性进行荟萃分析。
在8370条筛选记录中，50篇文章符合搜索标准，其中13项研究纳入荟萃分析。大多数研究在实验室环境（6
2%）中调查了氨基糖苷类抗生素接触对土壤微生物群的影响（93%），主要来自美国（32%）、中国（24%）、法国、瑞士和德国（8%）。少数研究调查了氨基糖苷类抗生素接触对疾病抑制的影响，因此被排除在荟萃分析之外。因此，我们的综述主要详细阐述了氨基糖苷类抗生素接触对土壤微生物群的微生物多样性和抗生素抗性的影响。总体而言，接触氨基糖苷类抗生素并未导致微生物多样性发生显著变化。然而，土壤用途、pH值和所用氨基糖苷类抗生素的类型可能是潜在的调节因素。此外，我们观察到平均7%的微生物种群对氨基糖苷类抗生素具有抗性，接触浓度与选择浓度之间的关系是一个潜在的调节因素。
目前的研究受到对氨基糖苷类抗生素接触、微生物群落动态和疾病抑制之间关系理解不足的限制，以及对研究较少的氨基糖苷类抗生素和关键混杂因素的数据不足的限制。目前的研究还表明，用于接触的抗生素浓度与抗性细菌的选择之间可能存在关系。这些发现强调了制定明智的抗生素管理政策以及进行严格、有针对性研究的必要性，以便更好地理解土壤因素和所用抗生素浓度对氨基糖苷类抗生素对土壤微生物群影响的关系。
